Aflatoxin Testing Solutions for Lab and On-Site Aflatoxin Detection

Aflatoxins are among the most potent fungal toxins to affect crops grown in warm and dry ambient conditions.

Most countries have established regulations to protect consumers from the harmful effects of aflatoxin intake. Such maximum levels are set for many agricultural commodities such as grains, grain by-products, feed and milk products. Separate regulations exist for total aflatoxin (aflatoxin B1, B2, G1, G2), aflatoxin B1 and aflatoxin M1.  

AgraStrip

AgraStrip®

Lateral Flow Devices

The AgraStrip® Aflatoxin test kits are ready-to-use lateral flow devices (LFD) for on-site testing. These allow a rapid analysis of a wide range of food and feed samples with assay times of 3-5 minutes.

The test kits are available in a qualitative or quantitative format. While qualitative LFDs can be read visually, quantitative tests are used with the AgraVision™ reader, to provide objective results and secure a consistent results documentation.

The test kits come complete with strips, antibody coated microwells, an assay buffer, pipette tips and Whirl-Pak® bags.

A complete overview can be found below. Click on the item numbers for more information.

Item No.Quantitation RangeLimit of DetectionNo. of Tests
AgraStrip® Aflatoxin
COKAS11002cut-off 4 ppb-24
COKAS1100U2cut-off 4 ppb-24
COKAS12001cut-off 10 ppb-24
COKAS1200U1cut-off 10 ppb-24
COKAS10001cut-off 20 ppb-24
COKAS1000U1cut-off 20 ppb-24
Item No.Quantitation RangeLimit of DetectionNo. of Tests
AgraStrip® Aflatoxin M1 for AgraVisionTM Reader
COKAS1500A0 - 600 ppt43 ppt24
Item No.Quantitation RangeLimit of DetectionNo. of Tests
AgraStrip® Aflatoxin for AgraVisionTM Reader
COKAS1600A0 - 100 ppb3.6 ppb24
Item No.Quantitation RangeLimit of DetectionNo. of Tests
AgraStrip® Aflatoxin 10 g extraction kit WATEX for AgraVisionTM Reader
COKAS1600WS10 - 500 ppb3.31 ppb24
Item No.Quantitation RangeLimit of DetectionNo. of Tests
AgraStrip® Aflatoxin 50 g extraction kit WATEX for AgraVisionTM Reader
COKAS1600W10 - 500 ppb3.31 ppb24

1USDA/GIPSA approved
2Japanese Department of Agriculture approved

Item No.Product
Readers for Result Quantification
EQASR1000AgraVisionTM with Printer
EQASR1003AgraVisionTM
EQASR1010AgraVisionTM Set-Up with Reader, Printer, Incubator, Tweezer

 

 

AgraQuant

AgraQuant®

ELISA Tests

AgraQuant® Aflatoxin test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented.

These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 20 - 65 minutes for up to 42 samples.

Test kits are in a ready-to-use format to reduce handling errors and come with a set of 5 standards, an antibody coated plate, a dilution plate, a conjugate, a substrate and a stop solution.

A complete overview can be found below. Click on the item numbers for more information.

Item No.Quantitation RangeLimit of DetectionNo. of Wells
AgraQuant® Aflatoxin
COKAQ10004 - 40 ppb3 ppb96
COKAQ10484 - 40 ppb3 ppb48
COKAQ110021 - 20 ppb1 ppb96
COKAQ114821 - 20 ppb1 ppb48
Item No.Quantitation RangeLimit of DetectionNo. of Wells
AgraQuant® Aflatoxin M1 Sensitive
COKAQ710025 - 500 ppt18 ppt96
COKAQ714825 - 500 ppt18 ppt48
Item No.Quantitation RangeLimit of DetectionNo. of Wells
AgraQuant® Aflatoxin M1 Plus
COKAQ7200100 - 2000 ppt89 ppt96
COKAQ7248100 - 2000 ppt89 ppt48
Item No.Quantitation RangeLimit of DetectionNo. of Wells
AgraQuant® Aflatoxin B1
COKAQ80002 - 50 ppb2 ppb96
COKAQ80482 - 50 ppb2 ppb48

2Japanese Department of Agriculture approved

Item No.ProductDescription
Readers for Result Quantification
EQOLE4700StatFax® ELISA Reader (Awareness Technology, Inc.)Stat Fax® 4700
EQOLE1495ELx800 UV Reader (BioTek Instruments Inc.)BioTek® ELx800 UV Absorbance Microplate Reader
EQOLE1408ChroMate® ELISA Reader (Awareness Technology, Inc.)ChroMate® 4300

 

 

FluoroQuant

FluoroQuant®

Fluorometry

The FluoroQuant® Aflatoxin test kits are fluorometric tests that can quantify aflatoxins in only a few minutes.

The kits are USDA/GIPSA approved and provide a wide quantitation range (from 0.5 up to 5000 µg/kg) without dilution steps. FluoroQuant® test kits are simple enough for on-site use in grain, feed and food handling facilities, but they are also appropriate for analytical laboratories.

A complete overview can be found below. Click on the item numbers for more information.

Item No.VersionLimit of DetectionNo. of Tests
FluoroQuant® Afla
COKFA10101-3 ppb25
Item No.VersionLimit of DetectionNo. of Tests
FluoroQuant® Afla Plus
COKFA30703acetonitrilefrom 0.5 ppb25
COKFA3070D3methanolfrom 0.6 ppb25
Item No.VersionLimit of DetectionNo. of Tests
FluoroQuant® Afla IAC
COKFA40101US Domestic Version3 ppb25
COKFA4020International Version3 ppb25

1USDA/GIPSA approved
3AOAC RI approved

Item No.Product
Readers for Result Quantifiation
EQFFM3000FQ Reader
EQFFM3010FQ Reader with Printer and Accessories

 

 

Aflatoxin Cleanup Columns

Aflatoxin columns range from simple one-step cleanup with MycoSep® and MultiSep® technology to immunoaffinity cleanup. These cleanup columns, some of which part of AOAC and CEN methods, ensure fast and reliable purification of your samples.

MycoSep

MycoSep® & MultiSep®

CleanUp Columns

MycoSep® and MultiSep® columns contain packing materials made of adsorbent mixes specially designed for all food and feed commodities, for fast cleanup within 30 seconds.

A complete overview can be found below. Click on the item numbers for more information.

Item No.ProductSpecificityNo. of Columns
MycoSep® 
COCMY2112MycoSep® 112 AflaZonAflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol25
COCMY22241MycoSep® 224 AflaZonAflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol25
COCMY2226MycoSep® 226 AflaZon+Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol25
COCMY22282MycoSep® 228 AflaPatAflatoxins B1, B2, G1 & G2, Patulin25
Item No.ProductSpecificityNo. of Columns
MultiSep® 
COCMU2224MultiSep® 224 AflaZonAflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol25
COCMU2226MultiSep®  226 AflaZon+Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol25
COCMU22272MultiSep® 228 AflaPatAflatoxins B1, B2, G1 & G2, Patulin25

1AOAC official method
2Japanese official method

MycoSpin

MycoSpin™

Cleanup Columns

Romer Labs MycoSpin™ 400 multi-mycotoxin two-step cleanup column in a spin format was developed to be used for most regulated mycotoxins to shorten the time-consuming cleanup process. Multiple mycotoxins can be analyzed simultaneously by LC-MS/MS.

Highly accurate LC-MS/MS results are achieved by combining the MycoSpin™ 400 cleanup with Biopure™ 13C isotope labeled standards.

Click on the item number below for more information.

Item No.ProductSpecificityNo. of Columns
MycoSpinTM
COCMY2400MycoSpin™ 400 MultitoxinAflatoxin B1, B2, G1 & G2, Trichothecenes Type A & B, Zearalenone, Ochratoxin A, Fumonisin B1, B2 & B325
StarLine

StarLineTM

Immunoaffinity Columns

StarLine™ immunoaffinity columns (IAC) are designed for simple and reliable cleanup before detection of mycotoxins in a broad range of different commodities. These columns can be used for the purification as well as concentration of mycotoxins prior to analysis by various techniques, such as HPLC, GC-MS, LC-MS, ELISA or direct fluorometry.

A complete overview can be found below. Click on the item numbers for more information.

Item No.SpecificityFormatNo. of Columns
AflaStar™ FIT
COIAC1001Aflatoxins B1, B2, G1 & G21 mL25
COIAC1501Aflatoxins B1, B2, G1 & G21 mL500
Item No.Quantitation RangeLimit of DetectionNo. of Tests
AflaStar™ R
COIAC1004Aflatoxins B1, B2, G1 & G23 mL25
COIAC1504Aflatoxins B1, B2, G1 & G23 mL500
Item No.Quantitation RangeLimit of DetectionNo. of Tests
AflaStar™ M1 R
COIAC1005Aflatoxin M13 mL25
Biopure

BiopureTM

Reference Materials

Under the brand Biopure™, Romer Labs offers the broadest range of aflatoxin reference materials currently available on the market. These high quality products are available in liquid “ready-to-use” and crystalline forms, and as single calibrants as well as calibrant mixtures.

The stars of the Biopure™ product line are the fully labeled 13C internal standards for the detection via mass spectrometry.

Matrix reference materials, also called quality control materials (QCM), are offered for method validation purposes and to control the performance of an analytical method.

Here you can download our complete Biopure Product List.

Analytical Service

 

Analytical Service

Romer Labs operates 3 fully accredited mycotoxin service laboratories on 3 continents – Europe, America and Asia, completing the extensive portfolio of mycotoxin test kits.

Mycotoxin testing services are offered in Austria, the United States and Singapore for a variety of sample matrices, including grains and other raw materials, and feed and food samples.

Both the ISO 9001 certification and ISO 17025 accreditation guarantee reliable and accurate results. We uphold our standards of accuracy and reliability by participating regularly in official proficiency testing programs.

More information on our analytical service offering can be found here.

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