Romer Labs Diagnostic GmbH operates an accredited analytical service laboratory in its headquarters in Tulln, approx. 40km west of Vienna, Austria under the brand name Quantas Analytics™
Internal quality assurance is guaranteed in line with ISO standards, reference samples are stored for three months and the lab participates in numerous proficiency tests covering all its analytical areas.
Quantas Analytics™ offers a broad portfolio of analysis in the areas of mycotoxins, food allergens and genetically modified organisms (GMOs).
|An overview of the analytical services we offer can be found in our online catalog |
For sample submission, please use the following sample submission forms:
|MYKOTOXINE (DEUTSCH)||LEBENSMITTELALLERGENE (DEUTSCH)||GVO (DEUTSCH)|
|MYCOTOXINS (ENGLISH)||FOOD ALLERGENS (ENGLISH)||GMO (ENGLISH)|
It is crucial to perform a representative sampling procedure for analytical purpose. It is the customer’s responsibility to provide a representative sample. Our analytical result only refers to the submitted sample.
Sampling and homogenization for the analysis of mycotoxins requires special detailed attention. Very low concentrations need to be determined and the distribution of mycotoxins is very inhomogenous due to the uneven growth of fungi. In the case of regulated mycotoxins, sampling and homogenization is described in European Commission regulation 401/2006 and their extensions 519/2014 and 178/2010. For sample preparation, we recommend Romer mills, which have been recommended and described in various guides including those by the AOAC, FAO and USDA.
Please send at least 2kg of unground materials (e.g. whole kernels) and/or 1kg for homogenous, finely ground materials (e.g. flour) for mycotoxin analysis.
For the food allergen analysis, please submit a 250g – 500g sample.
In the analysis of GMOs the sample size depends strongly on the degree of homogenization of the sample and the requirements for the results, i.e. the lower the limit of detection should be, the higher should be the amount or the rate of homogenization of the sample.
TURN AROUND TIMES
Usually the analytical result for allergens and mycotoxins is available after six working days (e.g. Monday to Monday in the following week) and five working days after receipt of the sample for GMO analysis (e.g. Monday to Friday).
Every Wednesday, we perform Mycotoxin analysis using ELISA. Depending on the available capacities, samples received by 8 a.m. that day will be tested on the same day.
We provide rapid analysis within two working days for a surcharge of 50% on the regular price for the analysis of mycotoxins by LC-MS/MS. Please check capacities with the lab-team.
Qualitative PCR analysis for GMOs can be performed within two business days of receipt of the samples (e.g. Monday to Wednesday), a 50% premium will be charged. A request for capacity before sample shipment is necessary.
Only those results are official that were obtained by an official sampling.
HPLC – High Performance Liquid Chromatography:
Chromatographic methods, such as High Performance Liquid Chromatography (HPLC) have a long tradition in analytical chemistry. Many official methods are based on chromatographic test procedures and are acknowledged as references. These methods can be used for a wide variety of applications. Disturbing interferences which can influence results can be reduced to a minimum compared to other technologies because of the chromatographic separation performed prior to detection. HPLC methods are mostly used for the analysis of mycotoxins, but there are also Gas Chromatography (GC) applications. A simple variation of liquid chromatography is Thin Layer Chromatography (TLC), which is rarely used any more in routine analysis. HPLC is often combined with UV-light detectors or fluorescence detectors for the detection of mycotoxins. The majority of the important mycotoxins can be detected at a very low sensitivity with these two detector types. In recent years, the combination of HPLC with mass spectrometers (LC-MS) has gained in importance. This method offers many possibilities and advantages. More and more analytes can be identified and quantified with a relatively small effort within a short time.
ELISA – Enzyme Linked Immuno Sorbent Assay
ELISA is a rapid quantitative detection method. Specific antibodies react with the mycotoxins or allergen proteins present in the sample. Simplified, the test induces a colour reaction the intensity of which correlates with the concentration of the analyte. The actual concentration of the mycotoxin or food allergen sought is determined by a photometric measurement and then calculated via a calibration. Complex materials (e.g. finished feed or processed foodstuffs) can produce false results when testing for mycotoxins using ELISA. Thus, we offer mycotoxin screening by ELISA for manufacturer validated commodities only (raw cereals). We recommend to always test silages or complex feed mixtures with our reference methods (HPLC).
ELISA is a suitable detection method also for complex foodstuffs for food allergen.
PCR – Polymerase Chain Reaction
PCR -analysis is an enzymatic test method, which allows the detection of specific gene-sequences after multiplication. A very small amount of DNA is copied million times by PCR and can then be detected by conventional analysis methods. A chromatographic separation of DNA -fragments of different sizes is achieved by gel electrophoresis. With the help of a reference marker, the size of the DNA fragments in the sample can be assigned to the gene-sequence sought. PCR has many application fields in medicine, but in food and feed industry it is an important method in the detection of GMOs.
Usually, a PCR test is qualitative, but positive GMO results are often also quantified to determine if a threshold has been exceeded.