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Clean-up columns (for mycotoxin analyses)


Principle of clean-up

Mycotoxins occur in various commodities, many of them are highly complex. Compared to the amounts of other components in agricultural commodities like carbohydrates, fatty acids or proteins, the amounts of mycotoxins present in a sample are incredibly low. Our clean-up columns must fulfill certain criteria:
  • Separate the toxin of interest from matrix interferences
  • Recover the toxin of interest quantitatively
  • Save time and costs

Our range of clean-up columns (single step multifunctional or immunoaffinity columns -IACs) for sample preparation prior to TLC, HPLC, GC, mass spectroscopy and fluorometry provides you with the most complete choice available. Our patented clean-up columns are used in AOAC Official Methods and a Performance Verified test kit and are well recognized in the scientific community.

Romer Labs® clean-up columns offer:

 

 

Star™ Immunoaffinity Columns (IACs)

StarTM immunoaffinity columns are reliable and user-friendly, designed for rapid and simple purification of mycotoxins from complex matrices, in cases where extremely low limits of detection are required (e.g. determination of toxins in baby food).

Working Principle
Immunoaffinity columns contain antibodies that selectively bind the toxin of interest from the crude extract. During the clean-up step, interferences can be washed from the column while the toxin is immobilized on the column. In the last step, the toxin is eluted from the column for determination purposes. Using this highly specific technology, almost all clean-up related issues can be solved.

Why Star Line?

Why use Star™ immunoaffinity columns?

  • The use of specific antibodies allow a most efficient clean-up.
  • inding of the analyte to the antibody allows the use of larger volumes of extract and the elution with small amounts of organic solvent, thus concentrating the sample and increasing the sensitivity of the analytical method.
  • Immunaffinity columns are part of many official methods for mycotoxin analysis.



For further information, register here >>

Star™ Immunoaffinity Columns Product List

Name Item No. specific for Volume
AflaStar™ FIT

COIAC1001
COIAC1501

 Aflatoxin B1, B2, G1, G2 1 mL
AflaStar™ R
 COIAC1004
COIAC1504
 Aflatoxin B1, B2, G1, G2
 3 mL
AflaStar™ M1 R COIAC1005 Aflatoxin M1 1 mL
OchraStar™ COIAC2000 Ochratoxin A 3 mL
OchraStar™ FIT COIAC2001
COIAC2501		 Ochratoxin A 1 mL
FumoniStar™ COIAC3000 Fumonisisn B1, B2, B3 3 mL
ZearaStar™ COIAC4000 Zearalenone, Zearalenols 1 mL
DONStar™ R COIAC5004
COIAC5504		 Deoxynivalenol (DON) 3 mL

MycoSep® Columns

MycoSep® columns remove analytical interferences from sample extracts in one quick, easy step, eliminating washing and elution steps found in solid phase extraction and affinity columns. This patented system allows multifunctional clean-up for HPLC, mass spectroscopy (MS), GC, fluorometry and TLC analysis of several different mycotoxins, with an optimal precision and accuracy.

Working Principle

MycoSep® columns allow a one-step clean-up within 30 seconds: The MycoSep® clean-up column is pushed into a test tube (containing the sample extract), forcing the extract to filter upwards through the packing material of the column. The interferences adhere to the chemical packing in the column and the purified extract, containing the analytes of interest, passes through a membrane (frit) to the surface of the column.

Why use MycoSep® clean-up?

  • If the alternative is liquid-liquid partition, the lower consumption of hazardous solvents and the huge reduction of working time are clear advantages of MycoSep® columns. The simple one step clean-up with MycoSep® excludes many sources of error.
  • If the alternative method is an immunoaffinity column specific only for deoxynivalenol, the MycoSep® clean-up is superior because it also allows the analysis of other type B trichothecenes, like Nivalenol, Fusarenon X, 3- and 15-Acetyldeoxynivalenol and type A trichothecenes, which is not possible after immunoaffinity clean-up. This method of analysis gives a more complete picture of the contamination of the sample with trichothecenes.
  • If the alternative is an immunoaffinity column, MycoSep® clean-up columns have the additional advantage of speed: clean-up takes only 30 seconds compared to approximately 30 minutes when clean-up is done with immunoaffinity columns.



For further information, register here >>

MycoSep® Column Product List

Name Item No. used for Comment
MycoSep® 113 Trich COCMY2113 Trichothecenes Type A & B for small sample volumes
MycoSep® 225 Trich COCMY2225 Trichothecenes Type A & B for grain and simple matrices
MycoSep® 227 Trich COCMY2227 Trichothecenes Type A & B for complex matrices
MycoSep® 112 AflaZon COCMY2112 Afaltoxins, Zearalenone for small sample volumes
MycoSep® 224 AflaZon COCMY2224 Afaltoxins, Zearalenone for grain and simple matrices
MycoSep® 226 AflaZon+ COCMY2226 Afaltoxins, Zearalenone for complex matrices
MycoSep® 228 AflaPat COCMY2228 Aflatoxins, Patulin for complex matrices
MycoSep® 229 Ochra COCMY2229 Ochratoxin A for complex matrices
MycoSep® 231 Fum COCMY2231 Fumonisins for maize
MycoSep® 150 Ergots
COCMY2150 Ergot Alkaloids for grain and simple matrices
MycoSep® 240 Mon COCMY2240 Moniliformin for grain and simple matrices

 

MultiSep® Columns

MycoSep® clean-up columns are also available in our MultiSep® syringe format, giving our customers higher flexibility in choosing their preferred format. MultiSep® columns are not only used for direct sample clean-up before HPLC, Mass spectrometry (MS), GC, etc. and they can also be used when additional clean-up is necessary.

Working Principle
Similar to the MycoSep® column format, the MultiSep® column format allows a one-step clean-up of sample extracts, containing the analyte of interest, within 30 seconds: The sample extract is applied on top of the MultiSep® clean-up column, allowing the extract to go through the packing material of the column by gravity. Alternatively, MultiSep® columns can be attached to SPE stations, thus allowing the application of vacuum or positive pressure to force the extract through the column material. The interferences adhere to the chemical packing in the column and the purified extract, containing the analytes of interest, can be easily be collected in a suitable vessel.

 

Why use MultiSep® clean-up?

  • If the alternative is liquid-liquid partition, the lower consumption of hazardous solvents and the huge reduction of working time are clear advantages of MultiSep® columns. The simple one-step clean-up procedure with MultiSep® columns excludes many sources of error.
  • If the alternative is an immunoaffinity column, MultiSep® clean-up columns have the additional advantage of speed: clean-up takes only 30 seconds compared to approximately 30 minutes when clean-up is done with immunoaffinity columns.
  • The MultiSep® column format in combination with SPE stations offers the opportunity to purify several different samples simultaneously.



For further information, register here >>

MultiSep® Columns Product List

Name Item No. used for Comment
MultiSep® 213 Trich COCMU2213 Trichothecenes Type A & B for complex matrices
in combination with MycoSep®
MultiSep® 216 Trich COCMU2216 Trichothecenes Type A & B for complex matrices
in combination with MycoSep®
MultiSep® 225 Trich COCMU2225 Trichothecenes Type A & B for grain and simple matrices
MultiSep® 227 Trich COCMU2227 Trichothecenes Type A & B for complex matrices
MultiSep® 224 AflaZon COCMU2224 Afaltoxins, Zearalenone for grain and simple matrices
MultiSep® 226 AflaZon+ COCMU2226 Afaltoxins, Zearalenone for complex matrices
MultiSep® 228 AflaPat COCMU2228 Aflatoxins, Patulin for complex matrices
MultiSep® 229 Ochra COCMU2229 Ochratoxin A for complex matrices
MycoSep® 211 Fum COCMU2211 Fumonisins for maize and maize-based feed and food