Aflatoxins mainly produced by the Aspergillus species are routinely detected with rapid tests (e.g. ELISA, LFD, Fluorometric tests) and reference methods (chromatography, e.g. LC-FLD or LC-MS/MS).
For a list of all products for aflatoxin analysis click here Aflatoxins >>
|ELISA for Aflatoxin Analysis|
When a rapid aflatoxin test result is needed AgraQuant® ELISA kits will deliver quantitative results within 10 to 20 minutes. An ELISA reader, such as the StatFax® or Chromate® Reader is applied to quantify the test kit’s results.
|Item No.||Product||No. of Wells|
|COKAQ1100||AgraQuant® ELISA Total Aflatoxins (1-20 ppb)||96|
|COKAQ1148||AgraQuant® ELISA Total Aflatoxins (1-20 ppb)||48|
|COKAQ1000||AgraQuant® ELISA Total Aflatoxins (4-40 ppb)||96|
|COKAQ1048||AgraQuant® ELISA Total Aflatoxins (4-40 ppb)||48|
|COKAQ8000||AgraQuant® ELISA Aflatoxin B1 (2-50 ppb)||96|
|COKAQ8048||AgraQuant® ELISA Aflatoxin B1 (2-50 ppb)||48|
|COKAQ7200||AgraQuant® ELISA Aflatoxin M1 Fast (100-2000 ppt)||96|
|COKAQ7248||AgraQuant® ELISA Aflatoxin M1 Fast (100-2000 ppt)||48|
|COKAQ7100||AgraQuant® ELISA Aflatoxin M1 Sensitive (25-500 ppt)||96|
|COKAQ7148||AgraQuant® ELISA Aflatoxin M1 Sensitive (25-500 ppt)||48|
|EQOLE1409||StatFax® 303 ELISA Reader (Awareness Technology, Inc.)|
|EQOLE1408||ChroMate® 4300 ELISA Reader (Awareness Technology, Inc.)|
Detailed Product Information on AgraQuant® Mycotoxin ELISA >>
Sometimes quick test results are needed in remote areas and, therefore, test systems for field use are essential. In such cases, AgraStrip® lateral flow device tests can rapidly analyse raw food and feed commodities in just 3 to 5 minutes without the need of any major equipment. LFD reader such as Romer Labs’ AgraVision™ is needed for a quantification of these test results.
|Item No.||Product||No. of Tests|
|COKAS1100||AgraStrip® Afla (4 ppb)||24|
|COKAS1100U||AgraStrip® Afla (4 ppb - incl. Whirl-Pak® bags)||24|
|COKAS1200||AgraStrip® Afla (10 ppb)||24|
|COKAS1200U||AgraStrip® Afla (10 ppb - incl. Whirl-Pak® bags)||24|
|COKAS1000||AgraStrip® Afla (20 ppb)||24|
|COKAS1000U||AgraStrip® Afla (20 ppb - incl. Whirl-Pak® bags)||24|
|COKAS1600A||AgraStrip Total Aflatoxin Quantitative (incl. Whirl-Pak® bags)||24|
|COKAS1500A||AgraStrip® Afla M1 Quantitative (0-600 ppt)||24|
|EQASR1003||AgraVision™ Reader (for AgraStrip® LFD Quantitative)|
Detailed Product Information on AgraStrip® Mycotoxins >>
A quick fluorometric test named FluoroQuant® can be applied to quantitatively measure aflatoxins within a wide detection range from 0.5 up to 5000 µg/kg in only a few minutes due to the natural fluoroscene of aflatoxins.
|Item No.||Product||No. of Tests|
|COKFA3070||FluoroQuant® Afla Plus (Acetonitrile)||25|
|COKFA3070D||FluoroQuant® Afla Plus (Methanol)||25|
|COKFA4010||FluoroQuant® Afla IAC (US Domestic Version)||25|
|COKFA4020||FluoroQuant® Afla IAC (International Version)||25|
|COKFA4030||FluoroQuant® Afla IAC (70/30 peanut)||25|
Detailed Product Information on FluoroQuant® Tests >>
A purification of the sample extract is essential for a reference test method such as HPLC, which is routinely done with a highly specific antibody based 3-step immunoaffinity cleanup or a rapid solid phase extraction based 1-step MycoSep® or MultiSep® cleanup.
|Item No.||Product||No. of Columns|
|COCMY2112||MycoSep®112 AflaZON (small size)||25|
|COIAC1001||AflaStar™ FIT IAC (1mL format)||25|
|COIAC1004||AflaStar™ R IAC||25|
|COIAC1005||AflarStar™ R M1 IAC||25|
Detailed Product Information on MycoSep® & MultiSep® Columns >>
Detailed Product Information on Starline™ IAC Columns >>
Each reference test method requires Biopure liquid calibrants, liquid calibrant mixes or solid calibrants for a calibration of the analytical method. Furthermore, these calibrants can be applied to fortify food and feed matrices with aflatoxins to simulate contaminated food or feed during internal method validations. Matrix reference materials and check sample materials are required for a final accuracy check.
Nowadays, more and more laboratories perform multitoxin analyses with LC-MS/MS systems. Matrix effects influence the analyte ionization in these high-end spectrometers, which can cause signal suppressions or enhancements. Internal standards, such as Biopure 13C-labeled calibrants, are required to correct these systematic errors. In addition, Biopure liquid calibrants, liquid calibrant mixes and solid calibrants are needed to perform system calibrations. And finally, naturally contaminated matrix reference materials and check sample materials are available to check for accuracy.