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Mycotoxins occur in various commodities, many of them highly complex. Compared to the amounts of other components in agricultural commodities like carbohydrates, fatty acids or proteins the amounts of mycotoxins present in a sample are incredibly low. Our clean up columns have to fulfill certain criteria:
- Recover the toxin of interest quantitatively
- Separate the toxin of interest in much higher amounts
- Save time and costs
Our range of clean up column (single step multifunctional or immunoaffinity columns) for sample preparation prior to TLC, HPLC or GC analysis gives you the most complete choice available, with solutions for multitoxin analysis using MS detection also available. Our patented clean up columns are AOAC approved and are well recognized in the scientific community.
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MycoSep® Columns
MycoSep® columns from Romer Labs® remove analytical interferences from food and feed extracts in one quick, easy step, eliminating washing and elution steps found in solid phase extraction and affinity columns. This patented system allows multifunctional clean up for HPLC, GC, Mass Spectroscopy, Fluorometry and TLC analysis of 17 different mycotoxins, with optimal precision and accuracy, and recovery of 85-100%.
MycoSep® columns allow a one-step clean up within 30 seconds: The MycoSep® clean up column is pushed into a test tube (containing the extract), forcing the extract to filter upwards through the packing material of the column. The interferences adhere to the chemical packing in the column and the purified extract passes through a membrane (frit) to the surface of the column.
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Mycosep® Columns
Aflatoxins
Trichothecenes
Ochratoxins
Zearalenone
Moniliformin
Fumonisin
Patulin
Details & Orders:
Distributors
Romer Labs® Units
For further product information, please Register or Login.
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Multisep® Columns
MycoSep® clean up columns are also available in our MultiSep® syringe format,
giving our customers higher flexibility in choosing their preferred format. MultiSep® columns are not only used for direct sample clean up, they can also be used when additional clean up is necessary.
MultiSep® columns are basic solid phase extraction (SPE) columns in the syringe format. These columns may be attached to SPE stations thus allowing the application of vacuum or positive pressure as well as working with gravity. They function either as 3 step (conditioning, adsorption, elution) columns for sample clean up, or as 2 step (conditioning, elution) additional sample clean up of complex matrices in combination with MycoSep® columns.
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Multisep® Columns
Aflatoxins
Trichothecenes
Ochratoxins
Zearalenone
Fumonisin
Patulin
Details & Orders:
Distributors
Romer Labs® Units
For further product information, please Register or Login.
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Star™ Immunoaffinity Columns
Star™ immunoaffinity columns (IACs) are reliable and user friendly, designed for rapid and simple purification of mycotoxins from complex matrices, in cases where extremely low limits of detection are required (e.g. determination of toxins in baby food).
Immunoaffinity columns contain antibodies that selectively bind the toxin of interest from the crude extract. During the clean up step interferences can be washed from the column while the toxin is immobilized on the column. In the last step the toxin can be eluted from the column for determination. Using this highly specific technology, most clean up problems can be solved.
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Star™ Columns
Aflatoxins
Ochratoxins
Zearalenone
Fumonisin
Details & Orders:
Distributors
Romer Labs® Units
For further product information, please Register or Login.
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Romer Labs® LC-MS/MS Packages
Fusarium -Toxin package contains all consumables for clean up, reference materials and fully 13C-labeled internal standards necessary to establish multi-mycotoxin determination on your LC-MS/MS instrument.
The Fusarium-Toxin Package enables you to set up a method for analyzing Type A Trichothecenes, Type B Trichothecenes and Zearalenone from various matrices.
It contains:
- 1 Box of MycoSep 226 AflaZon+ clean up columns
- Reference standard in acetonitrile (Type B Trichothecenes, Type A Trichothecenes and Zearalenone)
- Internal standard Zearalanone
- Internal standard U-[13C15]-Deoxynivalenol
- Internal standard U-[13C24]-T-2 Toxin
- Matrix reference material Corn containing Deoxynivalenol and Zearalenone
- Application note with method details
Multi-toxin package contains all consumables for clean up, reference materials and fully 13C-labeled internal standards necessary to establish multi-mycotoxin determination on your LC-MS/MS instrument.
The Multi-Toxin Package enables you to set up a method for analyzing Aflatoxins, Type A Trichothecenes, Type B Trichothecenes and Zearalenone from various matrices.
It contains:
- 1 Box of MycoSep 226 AflaZon+ clean up columns
- Reference standard in acetonitrile (Type B Trichothecenes, Type A Trichothecenes and Zearalenone)
- Reference standard in acetonitrile (Aflatoxin B1,B2, G1 and G2)
- Internal standard Zearalanone
- Internal standard U-[13C15]-Deoxynivalenol
- Internal standard U-[13C24]-T-2 Toxin
- Matrix reference material Corn containing Deoxynivalenol and Zearalenone
- Application note with method details
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Links
Star™ Columns
Aflatoxins
Ochratoxins
Zearalenone
Details & Orders:
Distributors
Romer Labs® Units
For further product information, please Register or Login.
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Application Table
Clean up steps necessary
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1 Step |
2 - 3 Steps |
 > 3 Steps
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Format
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MycoSep ® |
MultiSep ® |
MultiSep ® |
Immunoaffinity column |
B Trichothecenes
A Trichothecenes |
MycoSep® 113 Trich |
- |
- |
- |
MycoSep® 225 Trich+ |
MultiSep® 225 Trich |
MultiSep® 213 |
- |
MycoSep® 227 Trich+ |
MultiSep® 227 Trich+ |
MultiSep® 216 |
- |
Aflatoxin B1, B2, G1 and G2
Zearalenone |
MycoSep® 112 AflaZon |
- |
- |
AflaStar™ , ZearaStar™ |
MycoSep® 224 AflaZon |
MultiSep® 224 AflaZon |
- |
AflaStar™ , ZearaStar™ |
MycoSep® 226 AflaZon + |
MultiSep® 226 AflaZon + |
- |
AflaStar™ , ZearaStar™ |
Aflatoxin B1, B2, G1 and G2 Patulin |
MycoSep® 228 AflaPat |
MultiSep® 228 AflaPat |
- |
AflaStar™ |
Moniliformin |
MycoSep® 240 Mon |
- |
- |
- |
Ochratoxin A, B |
MycoSep® 229 Ochra |
MultiSep® 229 Ochra |
- |
OchraStar™ |
Fumonisins B1, B2 and B3 |
- |
- |
MultiSep® 211 Fum |
- |
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