MycoSep®/MultiSep® columns

The MultiSep® 211 Fum column is the only column in our portfolio following the classical 3 step solid phase extraction (SPE) principle. Fumonisins are adsorbed by the packing material while the extract drains through the pre-conditioned column. Interferences are washed from the column, and in the third step the fumonisins are eluted from the column. For further separation and detection purposes.

• MultiSep® 211 Fum column is designed for clean up of fumonisins from commodities like corn and corn-based feed and food.

The MycoSep®/MultiSep® 231 Fum columns are a one step clean up column developed for the purification of fumonisins.

• MycoSep®/MultiSep® 231 Fum columns has been validated for corn (maize).
• The MycoSep®/MultiSep® 231 Fum columns are very cost and time efficient alternative to immunaffinity columns. In cases when a very complex matrix has to be analysed e.g. cornflakes or others we recommend the use of our Star™ Immunoaffinity columns.

Why use MultiSep®/MycoSep® clean up?

• If the alternative is liquid-liquid partition the lower consumption of hazardous solvents and the huge reduction of working time is a clear advantage of MultiSep®/MycoSep® columns. The simple one step clean up with MultiSep®/MycoSep® excludes many sources for error.

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Star™ Immunoaffinity columns (IAC)

FumoniStar™ columns come in 3mL syringes (0.5ml gel bed volume). They contain immobilized monoclonal antibodies specific for fumonisins B1, B2 and B3. Their ability to endure high ratios of extraction solvents in the applied solution results in low volume application and reduced clean up time.

• FumoniStar™ columns are designed for the purification of fumonisins (FB1, FB2, FB3) from complex matrices such as cornflakes, polenta, etc.

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References:

Hammond B, Campbell K, Pilcher C, Degooyer T, Robinson A, McMillen B, Spangler S, Riordan S, Rice L and Richard J;
Lower Fumonisin Mycotoxin Levels in the Grain of Bt Corn Grown in the United States in 2000-2002;
Journal of Agricultural and Food Chemistry Vol. 52 (2004)