MycoSep®/MultiSep® columns

The MycoSep® 113 Trich, MycoSep®/MultiSep® 225 Trich and 227 Trich+ columns contain a proprietary mixture of various adsorbents and apply specifically to trichothecenes.

• MycoSep®/MultiSep® 227 Trich+ applies to complex matrices, like food products or finished feed.
  
• MycoSep®/MultiSep® 225 Trich is for grain and other simple matrices.
  
• MycoSep® 113 Trich is suitable for analytical procedures where only small amounts of extract are required.

Why use MultiSep®/MycoSep® clean up?

• If the alternative is liquid-liquid partition the lower consumption of hazardous solvents and the huge reduction of working time is a clear advantage of MultiSep®/MycoSep® columns. The simple one step clean up with MultiSep®/MycoSep® excludes many sources for error.



• If the alternative method is a Immunoaffinity DON column, the MycoSep®/MultiSep® clean up is superior because it allows also the analysis of other type B trichothecenes, like Nivalenol, Fusarenon X, 3- and 15-Acetyldeoxynivalenol and type A trichothecenes, which is not possible after immunoaffinity clean up. This way of analysis gives a more complete picture of the contamination of the sample with trichothecenes. The MultiSep®/MycoSep® clean up has the additional advantage of speed: clean up takes 30 seconds for the MycoSep®, 60 seconds for MultiSep® and about 30 minutes for an Immunoaffinity columns.

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Star^™ Immunoaffinity columns (IAC)

DonStar^™ columns come in 3mL syringes (0.5mL gel bead volume) and contain highly specific monoclonal antibodies for the purification and concentration of deoxynivalenol.

• DonStar^™ columns are designed for the purification of deoxynivalenol (vomitoxin) from a range of cereals, including corn, wheat, barley and from complex matrices such as DDGS and corn gluten products.
  

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References:

Trucksess MW, Ready DE, Pender MK, Ligmond CA, Wood GE, and Page SW,
Determination and survey of deoxynivalenol in white flour, whole wheat flour and bran;
J. AOAC Int., 79, 4 (1996) 883-887.

Malone BR, Humphrey CW, Romer TR, and Richard JL,
One-step solid phase extraction clean up and fluorometric analysis of deoxynivalenol in grains;
J. of AOAC Int., Vol. 87, No.2, (1998) 448-452.

Langseth W, Rundberget T,
Instrumental methods for determination of nonmacrocyclic trichothecenes in cereals, foodstuffs and cultures;
J. of Chrom. A, 815 (1998) 103-121.

Radova Z, Holadova K, Hajslova J,
Comparison of two clean up principles for determination of trichothecenes in grain extract;
J. of Chrom. A, 829 (1998) 259-267.

Mateo JJ, Llorens A, Mateo R, Jimenez M,
Critical study of and improvements in chromatographic methods for the analysis of type B trichothecenes;
J. of Chrom. A, 918 (2001) 99-112.

Mateo JJ, Mateo R, Hinojo MJ, Llorens A, Jimenez M,
Liquid chromatographic determination of toxigenic secondary metabolites produced by Fusarium strains;
J. of Chrom. A, 955 (2002) 245-256.

Razzazi-Fazeli E, Rabus B, Cecon B, Böhm J,
Simultaneous quantification of A-trichothecene mycotoxins in grains using LC-APCI-MS;
J. of Chrom. A, 968 (2002) 129-142.

Razzazi-Fazeli E, Böhm J, Jarukamjorn K, Zentek J,
Simultaneous determination of major B-trichothecenes and the de-epoxy-metabolite of deoxynivalenol in pig uringe and maize using high-performance liquid chromatography-mass spectrometry;
J. of Chrom. B, 796 (2003) 21-33.