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SH-AQ-Ochratoxin

AgraQuant® Ochratoxin ELISA test

ELISA Quantitative

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Grouped product items
Article numberLimit of detectionNumber of wellsAnalyteStatus
10002102 (COKAQ2000)
1.9 ppb
96Ochratoxin A
Limited availability
10002103 (COKAQ2048)
1.9 ppb
48Ochratoxin A
Limited availability

Description & Properties

AgraQuant® OTA test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented. These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 15 minutes for up to 42 samples. With the AgraQuant® ELISA, up to 6 mycotoxins can be analyzed from 1 extraction, substantially shortening overall time-to-result.
Properties
Quantitation range2-40 ppb
Limit of detection1.9 ppb
Limit of quantitation2 ppb
Technology/ApplicationELISA
Storage Temperature2°C - 8°C
Result typeQuantitative
Product/EnvironmentProduct Testing

Materials

Materials supplied in the standard product

  • antibody coated microwells in a microwell holder
  • non-coated dilution microwells
  • 5 vials of each ochratoxin A standard
  • 1 bottle of ochratoxin A conjugate
  • 1 bottle of substrate solution
  • 1 bottle of stop solution

Materials required but not supplied

  • Romer Series II® Mill or equivalent
  • balance - 400 g
  • blender or a tightly sealing jar with lid
  • 250mL conic flask with plug
  • graduated cylinder: 100mL
  • 100% methanol: ACS grade methanol
  • 70% methanol
  • distilled or de-ionized water
  • container for filtrate collection (e.g. flask or falcon tubes)
  • Whatman#1 filter paper or equivalent
  • filter funnel
  • 8-channel and single channel pipettes capable of pipetting 100µL and 200µL with tips
  • timer
  • wash bottle
  • absorbent paper towels
  • 3 reagent boats for 8-channel pipettes
  • microwell reader with a 450nm filter and an optional differential filter of 630nm or equivalent

Documents

10002102 (COKAQ2000):
10002103 (COKAQ2048):