Test Kits for the Accurate Detection of Histamine

Histamine is an organic nitrogenous compound which naturally occurs in the human body, where it acts as neurotransmitter and is involved in immune responses and the regulation of physiological functions.

In an allergic reaction, the immune system releases a high level of histamine, leading to inflammatory reactions which are responsible for typical allergic symptoms. Those who are intolerant to histamine will suffer from similar reactions from an oral intake of histamine.

Histamine as indicator for bacterial growth

Histamine is produced by the decomposition of histidine caused by the growth of certain bacteria in fish and some other food products. Fish meal produced from materials which have been allowed to degrade prior to being processed may contain high levels of histamine and can thus be toxic.

Fish containing high levels of histamine has been associated with many examples of poisoning commonly referred to as “scombroid poisoning,” a major health problem for consumers. Quality control measures designed to minimize the occurrence of scombrotoxic fish require the determination of histamine levels in the range of approximately 10 to 200 ppm.

Fast and highly sensitive histamine analysis

The Romer Labs test kit portfolio covers 2 highly sensitive ELISA methods for the quantitative analysis of histamine in different food matrices.

AgraQuant

AgraQuant®

ELISA Tests

AgraQuant® Histamine test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. These test kits have been validated for the analysis of histamine in fresh fish, fish meal, cheese, sausage, milk, wine and champagne.

The AgraQuant® Histamine Rapid test kits are specifically developed for the detection of histamine in fresh, canned and salted fish, as well as in fish meal and fish in oil. The workflow of these test kit has been optimized for fish samples and allows for a much faster assay procedure.

Both AgraQuant® Histamine test kits are competitive ELISAs in a ready-to-use format to reduce handling errors and come with a set of 8 standards, a histamine coated plate, an acylation reagent, an acylation buffer, a wash buffer, a histamine antiserum conjugate, a substrate and a stop solution.
Results are measured with a specific ELISA reader, such as the StatFax® or the BioTek® reader.

Click on the item numbers below for more information.

Item No. Quantitation Range Limit of Detection No. of Wells
AgraQuant® Histamine
10002017 0.5 - 50 ppb 0.15 ppb 96
Item No. Quantitation Range Limit of Detection No. of Wells
AgraQuant® Histamine Rapid
10002018 3 - 300 ppm 1 ppm 48

 

Item No. Product Description
Readers for Result Quantification
10002756 StatFax® ELISA Reader (Awareness Technology, Inc.) Stat Fax® 4700
10002740 800 TS Reader incl. Gen5 SW  (BioTek Instruments Inc.) BioTek® 800 TS Compact Microplate Reader (405, 450, 490 and 630 nm)
10002741 800 TS Reader (BioTek Instruments Inc.) BioTek® 800 TS Compact Microplate Reader(405, 450, 490 and 630 nm)
10002699 ChroMate® ELISA Reader (Awareness Technology, Inc.) ChroMate® 4300

 

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