Macadamia Nut Test Kits for Lab and On-Site Detection

Macadamia is a genus of trees indigenous to Australia, which is well known for its edible fruit, the macadamia nut. The seed, which is covered by a very hard shell, is frequently used as an ingredient in baked goods, confectioneries and breakfast cereals or consumed on its own.

Although the prevalence compared to other tree nuts is rather low, macadamia nuts can elicit severe allergic responses, especially in children. Hidden traces of macadamia nuts in food represent a critical problem for allergic individuals. The food industry is required to use sensitive macadamia nut detection methods to protect consumers from the consequences of potential cross contaminations.

Mandatory labeling of tree nuts in food

Tree nuts are considered one of the most frequent causes of food allergy, with macadamia nut allergy being among them. In Europe and the United States, the frequency of tree nut allergy as a whole is estimated to range between 0.2% and 0.5% both in children and adults. The only treatment for such an allergy is a strict and lifelong avoidance of macadamia nuts.

In order to protect consumers from the potentially fatal consequences of macadamia nut intake, allergen labeling is required by law in many countries. Macadamia nuts, along with other tree nuts, are regulated for example in the EU (Opens external link in new windowRegulation No 1169/201) and the U.S. (Opens external link in new windowFDA – FALCPA 2004).

Fast and highly sensitive macadamia nut analysis

The Romer Labs macadamia nut test kit portfolio covers ELISA and LFD methods for the quantitative and qualitative analysis of macadamia nuts in environmental samples, rinse waters and finished food products.



Lateral Flow Devices

The AgraStrip® Macadamia test kit is a ready-to-use lateral flow device (LFD) for on-site testing. This immunological test allows a rapid analysis of a wide range of food samples within only 11 minutes (including extraction).

AgraStrip® LFDs represent a fast and simple qualitative strip test method in which specific antibodies labeled with colloidal gold are used for visual color development at a test and control zone.

The test kit comes complete with strips, antibody coated incubation vials, extraction tubes, an extraction buffer, surface swabs and additional accessories.

Click on the item number below for more information.

Item No. No. of Tests
AgraStrip® Macadamia Nut
10002051 10





The AgraQuant® Plus Macadamia test kit is an accurate and reliable enzyme-linked immunosorbent assay (ELISA) specifically designed for a more efficient workflow. This quantitative test format uses unique extraction capsules that speed up the extraction of allergenic proteins, especially from processed matrices, to barely 1 minute.
Incubation times of the assay are reduced to 3 x 10 minutes, leading to a total assay time of 30 minutes for up to 38 samples.

AgraQuant® allergen test kits are sandwich ELISAs in a ready-to-use format to reduce handling errors and come with a set of 5 standards, an antibody coated plate, extraction capsules, a wash buffer, a conjugate, a substrate and a stop solution.

Click on the item numbers below for more information.

Item No. Quantitation Range Limit of Detection No. of Wells
AgraQuant® Plus Macadamia Nut
10002053 1 - 25 ppm 1 ppm 48


Item No. Product Description
Readers for Result Quantification
10002756 StatFax® ELISA Reader (Awareness Technology, Inc.) Stat Fax® 4700
10002740 800 TS Reader incl. Gen5 SW  (BioTek Instruments Inc.) BioTek® 800 TS Compact Microplate Reader (405, 450, 490 and 630 nm)
10002699 ChroMate® ELISA Reader (Awareness Technology, Inc.) ChroMate® 4300


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