Mustard Test Kits for Lab and On-Site Detection

Mustard refers to a group of plant species in the genera Brassica and Sinapis. The plant produces small round seeds, which are used as a spice. A common way to consume mustard is in the form of prepared mustard, which consists of ground mustard seeds mixed with water, vinegar and other ingredients.

Yellow, brown and black mustard are also frequently used as an ingredient in many food preparations. For mustard allergic people, these hidden allergens in food are a critical problem, as many mustard proteins are heat stable, making them resistant to different production processes.

Allergic individuals must strictly avoid the consumption of mustard, because even very low amounts can lead to severe allergic reactions. The food industry is required to use sensitive mustard test systems, as cross contaminations may occur during the food production process.

Mandatory labeling of mustard in food

Globally, mustard is not considered one of the most important causes of food allergy. But in regions where a lot of mustard is produced and consumed (i.e. France) the prevalence of mustard allergies is relatively high. The only treatment for such an allergy is a strict and lifelong avoidance of mustard.

In order to protect consumers from the potentially fatal consequences of mustard consumption, allergen labeling is required by law in many countries. Mustard is regulated by law in Europe (Opens external link in new windowRegulation (EU) No 1169/201) and Canada among others.

Fast and highly sensitive mustard analysis

The Romer Labs mustard test kit portfolio covers ELISAs and LFDs for the quantitative and qualitative analysis of mustard in environmental samples, rinse waters and finished food products.



Lateral Flow Devices

The AgraStrip® Mustard test kit is a ready-to-use lateral flow device (LFD) for on-site testing. This immunological test allows a rapid analysis of a wide range of food samples within only 11 minutes (including extraction).

AgraStrip® LFDs represent a fast and simple qualitative strip test method in which specific antibodies labeled with colloidal gold are used for visual color development at a test and control zone.

The test kit comes complete with strips, antibody coated incubation vials, extraction tubes, an extraction buffer, surface swabs and additional accessories.

Click on the item number below for more information.

Item No. No. of Tests
AgraStrip® Mustard
10002068 10



The AgraQuant® Mustard test kit is an accurate and reliable enzyme-linked immunosorbent assay (ELISA) in a quantitative format. Results are measured with a specific ELISA reader, such as the StatFax® or the BioTek® reader.

The AgraQuant® Plus Mustard test kit is specifically designed for a more efficient workflow. This test format uses unique extraction capsules that speed up the extraction of allergenic proteins, especially from processed matrices, to barely 1 minute.
Incubation times of the assay are reduced to 3 x 10 minutes, leading to a total assay time of 30 minutes for up to 38 samples.

Both AgraQuant® Mustard test kits are sandwich ELISAs in a ready-to-use format to reduce handling errors and come with a set of 5 standards, extraction solution /capsules, an antibody coated plate, a wash buffer, a conjugate, a substrate and a stop solution.

Click on the item numbers below for more information.

Item No. Quantitation Range Limit of Detection No. of Wells
AgraQuant® Mustard
10002070 2 - 60 ppm 1 ppm 48
Item No. Quantitation Range Limit of Detection No. of Wells
AgraQuant® Plus Mustard
10002072 1 - 25 ppm 0.5 ppm 48


Item No. Product Description
Readers for Result Quantification
10002756 StatFax® ELISA Reader (Awareness Technology, Inc.) Stat Fax® 4700
10002740 800 TS Reader incl. Gen5 SW  (BioTek Instruments Inc.) BioTek® 800 TS Compact Microplate Reader (405, 450, 490 and 630 nm)
10002699 ChroMate® ELISA Reader (Awareness Technology, Inc.) ChroMate® 4300


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