Aflatoxin Testing Solutions for Lab and On-Site Aflatoxin Detection
Aflatoxins are among the most potent fungal toxins to affect crops grown in warm and dry ambient conditions.
Most countries have established regulations to protect consumers from the harmful effects of aflatoxin intake. Such maximum levels are set for many agricultural commodities such as grains, grain by-products, feed and milk products. Separate regulations exist for total aflatoxin (aflatoxin B1, B2, G1, G2), aflatoxin B1 and aflatoxin M1.
Romer Labs aflatoxin testing solutions:
AgraStrip®
Lateral Flow Devices
The AgraStrip® test kits are ready-to-use lateral flow devices (LFD) for on-site testing. These allow a rapid analysis of a wide range of food and feed samples with an assay time as low as 3 minutes.
The test kits are available in a qualitative or quantitative format. While qualitative LFDs can be read visually, quantitative tests are used with the AgraVision™ and AgraVision™ Pro readers to provide objective results and secure a consistent results documentation.
A complete overview can be found below. Click on the item numbers for more information.
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| AgraStrip® Pro Total Aflatoxin WATEX® | |||
|---|---|---|---|
| 10006322 | 0 – 460 ppb | 2/1 ppb* | 40 |
| AgraStrip® Aflatoxin | |||
|---|---|---|---|
| 100021292 (excl. Whirl-Pak® bag) | cut-off 4 ppb | - | 24 |
| 100021302 | cut-off 4 ppb | - | 24 |
| 100021311 (excl. Whirl-Pak® bag) | cut-off 10 ppb | - | 24 |
| 100021321 | cut-off 10 ppb | - | 24 |
| 100021261 (excl. Whirl-Pak® bag) | cut-off 20 ppb | - | 24 |
| 100021261 | cut-off 20 ppb | - | 24 |
* High-sensitive method for aflatoxin available
1 USDA/FGIS approved
2 Japanese Department of Agriculture approved
4 AgraVisionTM Reader
AgraQuant®
ELISA Tests
AgraQuant® Aflatoxin test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented.
These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 20 - 65 minutes for up to 42 samples.
Test kits are in a ready-to-use format to reduce handling errors and come with a set of 5 standards, an antibody coated plate, a dilution plate, a conjugate, a substrate and a stop solution.
A complete overview can be found below. Click on the item numbers for more information.
| Item No. | Quantitation Range | Limit of Detection | No. of Wells |
| AgraQuant® Aflatoxin | |||
|---|---|---|---|
| 10002098 | 4 - 40 ppb | 3 ppb | 96 |
| 10002099 | 4 - 40 ppb | 3 ppb | 48 |
| 100021002 | 1 - 20 ppb | 1 ppb | 96 |
| 100021012 | 1 - 20 ppb | 1 ppb | 48 |
| Item No. | Quantitation Range | Limit of Detection | No. of Wells |
| AgraQuant® Aflatoxin M1 Sensitive | |||
|---|---|---|---|
| 10002116 | 25 - 500 ppt | 18 ppt (fresh milk) | 96 |
| 10002117 | 25 - 500 ppt | 18 ppt (fresh milk) | 48 |
| AgraQuant® Aflatoxin M1 High Sensitivity | |||
|---|---|---|---|
| 10002120 | 5 - 100 ppt | 2.9 ppt (raw milk) | 96 |
| 10002121 | 5 - 100 ppt | 2.9 ppt (raw milk) | 48 |
| AgraQuant® Aflatoxin B1 | |||
|---|---|---|---|
| 10002122 | 2 - 50 ppb | 2 ppb | 96 |
| 10002123 | 2 - 50 ppb | 2 ppb | 48 |
2Japanese Department of Agriculture approved
| Item No. | Product | Description |
| Readers for Result Quantification | ||
|---|---|---|
| 10002756 | StatFax® ELISA Reader (Awareness Technology, Inc.) | Stat Fax® 4700 |
| 10002740 | 800 TS Reader incl. Gen5 SW (BioTek Instruments Inc.) | BioTek® 800 TS Compact Microplate Reader (405, 450, 490 and 630 nm) |
| 10002699 | ChroMate® ELISA Reader (Awareness Technology, Inc.) | ChroMate® 4300 |
Aflatoxin Cleanup Columns
Aflatoxin columns range from simple one-step cleanup with MycoSep® and MultiSep® technology to immunoaffinity cleanup. These cleanup columns, some of which part of AOAC and CEN methods, ensure fast and reliable purification of your samples.
MycoSep® & MultiSep®
CleanUp Columns
MycoSep® and MultiSep® columns contain packing materials made of adsorbent mixes specially designed for all food and feed commodities, for fast cleanup within 30 seconds.
A complete overview can be found below. Click on the item numbers for more information.
| Item No. | Product | Specificity | No. of Columns |
| MycoSep® | |||
|---|---|---|---|
| 10001948 | MycoSep® 112 AflaZon | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| 100019511 | MycoSep® 224 AflaZon | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| 10001953 | MycoSep® 226 AflaZon+ | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| 100019552 | MycoSep® 228 AflaPat | Aflatoxins B1, B2, G1 & G2, Patulin | 25 |
| MultiSep® | |||
|---|---|---|---|
| 10001939 | MultiSep® 224 AflaZon | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| 10001941 | MultiSep® 226 AflaZon+ | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| 100019422 | MultiSep® 228 AflaPat | Aflatoxins B1, B2, G1 & G2, Patulin | 25 |
1AOAC official method
2Japanese official method
MycoSpin®
Cleanup Columns
Romer Labs MycoSpin® 400 multi-mycotoxin two-step cleanup column in a spin format was developed to be used for most regulated mycotoxins to shorten the time-consuming cleanup process. Multiple mycotoxins can be analyzed simultaneously by LC-MS/MS.
Highly accurate LC-MS/MS results are achieved by combining the MycoSpin® 400 cleanup with Biopure™ 13C isotope labeled standards.
Click on the item number below for more information.
| Item No. | Product | Specificity | No. of Columns |
| MycoSpin® | |||
|---|---|---|---|
| 10001961 | MycoSpin® 400 Multitoxin | Aflatoxin B1, B2, G1 & G2, Trichothecenes Type A & B, Zearalenone, Ochratoxin A, Fumonisin B1, B2 & B3 | 25 |
StarLineTM
Immunoaffinity Columns
StarLine™ immunoaffinity columns (IAC) are designed for simple and reliable cleanup before detection of mycotoxins in a broad range of different commodities. These columns can be used for the purification as well as concentration of mycotoxins prior to analysis by various techniques, such as HPLC, GC-MS, LC-MS, ELISA or direct fluorometry.
A complete overview can be found below. Click on the item numbers for more information.
| Item No. | Specificity | Format | No. of Columns |
| AflaStar™ FIT | |||
|---|---|---|---|
| 10001962 | Aflatoxins B1, B2, G1 & G2 | 1 mL | 25 |
| 10001965 | Aflatoxins B1, B2, G1 & G2 | 1 mL | 500 |
| AflaStar™ R | |||
|---|---|---|---|
| 10001963 | Aflatoxins B1, B2, G1 & G2 | 3 mL | 25 |
| 10001966 | Aflatoxins B1, B2, G1 & G2 | 3 mL | 500 |
| AflaStar™ M1 R | |||
|---|---|---|---|
| 10001964 | Aflatoxin M1 | 3 mL | 25 |
StarLine™
Immunoaffinity Columns
MultiStar™ immunoaffinity columns belong to the StarLine™ portfolio of immunoaffinity column (IAC). These columns are designed for the reliable clean-up of samples to simplify the detection of several mycotoxins at once in a broad range of different commodities.
MultiStar™ immunoaffinity columns combine the high purification potential of an immunoaffinity column with the convenience and power of multi-mycotoxin analysis, allowing for all regulated mycotoxins to be determined simultaneously in a single run.
| Item no. | Analytes | No. of columns |
| MultiStar™ - Immunoaffinity Column | ||
|---|---|---|
| 10005407 | Aflatoxins, OTA, ZON, DON, FUM, T2 and HT2 | 25 |
BiopureTM
Reference Materials
Under the brand Biopure™, Romer Labs offers the broadest range of aflatoxin reference materials currently available on the market. These high quality products are available in liquid “ready-to-use” and crystalline forms, and as single calibrants as well as calibrant mixtures.
The stars of the Biopure™ product line are the fully labeled 13C internal standards for the detection via mass spectrometry.
Quality control materials (QCM) are offered for method validation purposes and to control the performance of an analytical method.
Here you can download our complete Biopure Product List.
Analytical Service
Romer Labs operates 4 fully accredited mycotoxin service laboratories on 3 continents – Europe, America and Asia, completing the extensive portfolio of mycotoxin test kits.
Mycotoxin testing services are offered in Austria, the United States, Singapore and China for a variety of sample matrices, including grains and other raw materials, and feed and food samples.
Both the ISO 9001 certification and ISO 17025 accreditation guarantee reliable and accurate results. We uphold our standards of accuracy and reliability by participating regularly in official proficiency testing programs. Our lab in China is CMA-certified.
More information on our analytical service offering can be found here.
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The major fungus producing aflatoxins is Aspergillus flavus... more
