Ochratoxin Testing Solutions
Ochratoxins are potent fungal toxins that affect unprocessed and processed food and feed mainly under hot and humid storage conditions.
Ochratoxins are known to primarily act as kidney toxins and are a carcinogen to rodents. The toxin is formed by the Aspergillus and Penicillium species and mainly affects processed and unprocessed crops, coffee, wine and dried grapes. Ochratoxin is mostly produced during storage under conditions that favor mold growth – that is, warm and humid conditions with moisture levels above 14% and temperatures above 20°C.
Countries have established regulations to protect consumers from the harmful effects of ochratoxin intake. The European Union for instance has established maximum levels for food and guidance levels for feed. China has established allowable levels of ochratoxin in feed. Other countries that have established regulations are Singapore, Malaysia, Korea, Indonesia and China.
Romer Labs ochratoxin testing solutions:
AgraStrip®
Lateral Flow Devices
The AgraStrip® test kits are ready-to-use lateral flow devices (LFD) for on-site testing. These allow a rapid analysis of a wide range of food and feed samples with a assay time of 3 minutes.
The test kits are available in a qualitative or quantitative format. While qualitative LFDs can be read visually, quantitative tests are used with the AgraVision™ reader, to provide objective results and secure a consistent results documentation.
The test kits come complete with strips, antibody coated microwells, an assay buffer, pipette tips and Whirl-Pak® bags.
Click on the item numbers below for more information.
| Item No. | Quantitation Range | Limit of Detection | No. of tests |
| AgraStrip® Ochratoxin A WATEX® | |||
|---|---|---|---|
| 10006160 | 0 - 100 ppb | 4 ppb | 24 |
| AgraStrip® Pro Ochratoxin A WATEX® | |||
| 10006332 | 0 -100 ppb | 0.5 ppb | 40 |
AgraQuant®
ELISA Tests
AgraQuant® OTA test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented.
These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 15 minutes for up to 42 samples.
Test kits are in a ready-to-use format to reduce handling errors and come with a set of 5 standards, an antibody coated plate, a dilution plate, a conjugate, a substrate and a stop solution.
Click on the item numbers below for more information.
| Item No. | Quantitation Range | Limit of Detection | No. of Wells |
| AgraQuant® Ochratoxin | |||
|---|---|---|---|
| 10002102 | 2 - 40 ppb | 1.9 ppb | 96 |
| 10002103 | 2 - 40 ppb | 1.9 ppb | 48 |
| Item No. | Product | Description |
| Readers for Result Quantification | ||
|---|---|---|
| 10002756 | StatFax® ELISA Reader (Awareness Technology, Inc.) | Stat Fax® 4700 |
| 10002740 | 800 TS Reader incl. Gen5 SW (BioTek Instruments Inc.) | BioTek® 800 TS Compact Microplate Reader (405, 450, 490 and 630 nm) |
| 10002741 | 800 TS Reader (BioTek Instruments Inc.) | BioTek® 800 TS Compact Microplate Reader(405, 450, 490 and 630 nm) |
| 10002699 | ChroMate® ELISA Reader (Awareness Technology, Inc.) | ChroMate® 4300 |
Cleanup Columns
OTA columns range from simple one-step cleanup with MycoSep® and MultiSep® technology to immunoaffinity cleanup. These cleanup columns ensure fast and reliable purification of your samples.
MycoSep® & MultiSep®
Cleanup Columns
MycoSep® and MultiSep® columns contain packing materials made of adsorbent mixes specially designed for all food and feed commodities, for a fast cleanup within 30 seconds.
Click on the item numbers below for more information.
| Item No. | Product | Specificity | No. of Columns |
| MycoSep® | |||
|---|---|---|---|
| 10001956 | MycoSep® 229 Ochra | Ochratoxin A | 25 |
| Item No. | Product | Specificity | No. of Columns |
| MultiSep® | |||
|---|---|---|---|
| 10001944 | MultiSep® 229 Ochra | Ochratoxin A | 25 |
MycoSpin®
Cleanup Columns
Romer Labs MycoSpin® 400 multi-mycotoxin two-step cleanup column in a spin format was developed to be used for most regulated mycotoxins to shorten the time-consuming cleanup process. Multiple mycotoxins can be analyzed simultaneously by LC-MS/MS.
Highly accurate LC-MS/MS results are achieved by combining the MycoSpin® 400 cleanup with Biopure™ 13C isotope labeled standards.
Click on the item number below for more information.
| Item No. | Product | Specificity | No. of Columns |
| MycoSpin® | |||
|---|---|---|---|
| 10001961 | MycoSpin® 400 Multitoxin | Aflatoxin B1, B2, G1 & G2, Trichothecenes Type A & B, Zearalenone, Ochratoxin A, Fumonisin B1, B2 & B3 | 25 |
StarLineTM
Immunoaffinity Columns
StarLine™ immunoaffinity columns (IAC) are designed for simple and reliable cleanup prior to ochratoxin detection in a broad range of different commodities. The columns can be used to purify and concentrate the OTA level prior to analysis by various techniques, such as HPLC, GC-MS, LC-MS, ELISA or direct fluorometry.
Click on the item number for more information.
| Item No. | Specificity | Format | No. of Columns |
| OchraStar™ R | |||
|---|---|---|---|
| 10001970 | Ochratoxin A | 3 mL | 25 |
StarLine™
Immunoaffinity Columns
MultiStar™ immunoaffinity columns belong to the StarLine™ portfolio of immunoaffinity column (IAC). These columns are designed for the reliable clean-up of samples to simplify the detection of several mycotoxins at once in a broad range of different commodities.
MultiStar™ immunoaffinity columns combine the high purification potential of an immunoaffinity column with the convenience and power of multi-mycotoxin analysis, allowing for all regulated mycotoxins to be determined simultaneously in a single run.
| Item no. | Analytes | No. of columns |
| MultiStar™ - Immunoaffinity Column | ||
|---|---|---|
| 10005407 | Aflatoxins, OTA, ZON, DON, FUM, T2 and HT2 | 25 |
BiopureTM
Reference Materials
Under the brand Biopure™, Romer Labs offers a broad range of ochratoxin reference materials. These high quality products are available in liquid “ready-to-use” and crystalline forms, and as single calibrants as well as calibrant mixtures.
The stars of the Biopure™ product line are the fully labeled 13C internal standards for mass spectrometry analysis.
Quality control materials (QCM) are offered for method validation purposes and to control the performance of an analytical method.
Here you can download our complete Biopure Product List.
Analytical Service
Romer Labs operates 3 fully accredited mycotoxin service laboratories on 3 continents – Europe, America and Asia -, completing the extensive portfolio of mycotoxin test kits.
Mycotoxin testing services are offered in Austria, the United States and Singapore for a variety of sample matrices, including grains and other raw materials, and feed and food samples.
Both the ISO 9001 certification and ISO 17025 accreditation guarantee reliable and accurate results. We uphold our standards of accuracy and reliability by participating regularly in official proficiency testing programs.
More information on our analytical service offering can be found here.
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The primary producers of ochratoxin are Aspergillus ochraceus and Penicillium verrucosum... more
