Zearalenone Testing Solutions for Lab and On-Site Zearalenone Detection

Zearalenone (also known as ZON, ZEN or ZEA) is a potent fungal toxin that affects crops grown or stored in cool and moist conditions.

Zearalenone is known to cause estrogenic effects such as precocious development in swine. The toxin is formed by the Fusarium species and mainly affects corn, wheat, sorghum, barley and rye. Such Fusarium species are known to co-produce other toxins such as deoxynivalenol.

Many countries have established regulations to protect consumers from the harmful effects of zearalenone intake. The European Union has established maximum levels for food and recommendations for feed. Other countries that have established regulations are Korea, Japan and China.

AgraStrip

AgraStrip®

Lateral Flow Devices

The AgraStrip® Zearalenone test kit is a ready-to-use lateral flow devices (LFD) for on-site testing. These allow a rapid analysis of a wide range of food and feed samples with assay times of 3 minutes.

The test kit is available in a quantitative format. Quantitative tests are used with the AgraVision™ reader, to provide objective results and secure a consistent results documentation.

The test kit comes complete with strips, antibody coated microwells, an assay buffer, pipette tips and Whirl-Pak® bags.

Click on the item number below for more information.

Item No.Quantitation RangeLimit of DetectionNo. of Tests
AgraStrip® WATEX® Zearalenone 
COKAS5000WS3 (10g extraction)0 - 1000 ppb30 ppb 24
COKAS5000W1,3 (50g extraction)0 - 1000 ppb30 ppb24

1USDA/GIPSA approved
3 AgraVisionTM Reader

AgraQuant

AgraQuant®

ELISA Tests

The AgraQuant® ZON test kit is an accurate and reliable enzyme-linked immunosorbent assay (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented.

This ELISA test kit is the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 15 minutes for up to 42 samples.

Test kit is in a ready-to-use format to reduce handling errors and comes with a set of 5 standards, an antibody coated plate, a dilution plate, a conjugate, a substrate and a stop solution.

Click on the item numbers below for more information.

Item No.Quantitation RangeLimit of DetectionNo. of Wells
AgraQuant® Zearalenone Plus
COKAQ510025 - 1000 ppb20 ppb96
COKAQ514825 - 1000 ppb20 ppb48

 

 

Item No.ProductDescription
Readers for Result Quantification
EQOLE4700StatFax® ELISA Reader (Awareness Technology, Inc.)Stat Fax® 4700
EQOLE1495ELx800 UV Reader (BioTek Instruments Inc.)BioTek® ELx800 UV Absorbance Microplate Reader
EQOLE1408ChroMate® ELISA Reader (Awareness Technology, Inc.)ChroMate® 4300

 

 

Zearalenone Cleanup Columns

ZON columns range from simple one-step cleanup with MycoSep® and MultiSep® technology to immunoaffinity cleanup. These cleanup columns, some of which part of AOAC and CEN methods, ensure fast and reliable purification of your samples.

MycoSep

MycoSep® & MultiSep®   

Cleanup Columns

MycoSep® and MultiSep® columns contain packing materials made of adsorbent mixes specially designed for all food and feed commodities, for a fast cleanup within 30 seconds.

Click on the item numbers below for more information.

Item No.ProductSpecificityNo. of Columns
MycoSep® 
COCMY2112MycoSep® 112  AflaZon 

Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol

25
COCMY22242MycoSep® 224  AflaZon Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol25
COCMY2226MycoSep® 226  AflaZon+Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol25
Item No.ProductSpecificityNo. of Columns
MultiSep® 
COCMU2224MultiSep® 224 AflaZonAflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol25
COCMU2226MultiSep® 226 AflaZon+Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol25

2AOAC official method

MycoSpin

MycoSpin®

Cleanup Columns

Romer Labs MycoSpin® 400 multi-mycotoxin two-step cleanup column in a spin format was developed to be used for most regulated mycotoxins to shorten the time-consuming cleanup process. Multiple mycotoxins can be analyzed simultaneously by LC-MS/MS.

Highly accurate LC-MS/MS results are achieved by combining the MycoSpin® 400 cleanup with Biopure™ 13C isotope labeled standards.

Click on the item number below for more information.

Item No.ProductSpecificityNo. of Columns
MycoSpin®
COCMY2400MycoSpin® 400 MultitoxinAflatoxin B1, B2, G1 & G2, Trichothecenes Type A & B, Zearalenone, Ochratoxin A, Fumonisin B1, B2 & B325
StarLine

StarLineTM

Immunoaffinity Columns

StarLine™ immunoaffinity columns are designed for simple and reliable cleanup before detection of zearalenone in a broad range of different commodities. Those IAC can be used for the purification as well as concentration of mycotoxins prior to analysis by various techniques, such as HPLC, GC-MS, LC-MS, ELISA or direct fluorometry. 

Click on the item number below for more information.

Item No.SpecificityFormatNo. of Columns
ZearaStar™ R
COIAC4004Zearalenone3 mL25
Biopure

BiopureTM

Reference Materials

Under the brand Biopure™, Romer Labs offers a broad range of zearalenone reference materials. These high quality products are available in liquid “ready-to-use” and crystalline forms, and as single calibrants as well as calibrant mixtures.

The stars of the Biopure™ product line are the fully labeled 13C internal standards for mass spectrometry analysis.

Quality control materials (QCM) are offered for method validation purposes and to control the performance of an analytical method.

Here you can download our complete Biopure Product List.

Analytical Service

 

Analytical Service

Romer Labs operates 3 fully accredited mycotoxin service laboratories on 3 continents – Europe, America and Asia -, completing the extensive portfolio of mycotoxin test kits.

Mycotoxin testing services are offered in Austria, the United States and Singapore for a variety of sample matrices, including grains and other raw materials, and feed and food samples.

Both the ISO 9001 certification and ISO 17025 accreditation guarantee reliable and accurate results. We uphold our standards of accuracy and reliability by participating regularly in official proficiency testing programs.

More information on our analytical service offering can be found here.

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