Zearalenone Testing Solutions for Lab and On-Site Zearalenone Detection
Zearalenone (also known as ZON, ZEN or ZEA) is a potent fungal toxin that affects crops grown or stored in cool and moist conditions.
Zearalenone is known to cause estrogenic effects such as precocious development in swine. The toxin is formed by the Fusarium species and mainly affects corn, wheat, sorghum, barley and rye. Such Fusarium species are known to co-produce other toxins such as deoxynivalenol.
Many countries have established regulations to protect consumers from the harmful effects of zearalenone intake. The European Union has established maximum levels for food and recommendations for feed. Other countries that have established regulations are Korea, Japan and China.
Romer Labs zearalenone testing solutions:
AgraStrip®
Lateral Flow Devices
The AgraStrip® Zearalenone test kit is a ready-to-use lateral flow devices (LFD) for on-site testing. These allow a rapid analysis of a wide range of food and feed samples with assay times of 3 minutes.
The test kit is available in a quantitative format. Quantitative tests are used with the AgraVision™ reader, to provide objective results and secure a consistent results documentation.
The test kit comes complete with strips, antibody coated microwells, an assay buffer, pipette tips and Whirl-Pak® bags.
Click on the item number below for more information.
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| AgraStrip® WATEX® Zearalenone | |||
|---|---|---|---|
| 100021533 (10g extraction) | 0 - 1000 ppb | 30 ppb | 24 |
| 100021521,3 (50g extraction) | 0 - 1000 ppb | 30 ppb | 24 |
1USDA/FGIS approved
3 AgraVisionTM Reader
AgraQuant®
ELISA Tests
The AgraQuant® ZON test kit is an accurate and reliable enzyme-linked immunosorbent assay (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented.
This ELISA test kit is the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 15 minutes for up to 42 samples.
Test kit is in a ready-to-use format to reduce handling errors and comes with a set of 5 standards, an antibody coated plate, a dilution plate, a conjugate, a substrate and a stop solution.
Click on the item numbers below for more information.
| Item No. | Quantitation Range | Limit of Detection | No. of Wells |
| AgraQuant® Zearalenone Plus | |||
|---|---|---|---|
| 10002111 | 25 - 1000 ppb | 20 ppb | 96 |
| 10002112 | 25 - 1000 ppb | 20 ppb | 48 |
| Item No. | Product | Description |
| Readers for Result Quantification | ||
|---|---|---|
| 10002756 | StatFax® ELISA Reader (Awareness Technology, Inc.) | Stat Fax® 4700 |
| 10002740 | 800 TS Reader incl. Gen5 SW (BioTek Instruments Inc.) | BioTek® 800 TS Compact Microplate Reader (405, 450, 490 and 630 nm) |
| 10002741 | 800 TS Reader (BioTek Instruments Inc.) | BioTek® 800 TS Compact Microplate Reader(405, 450, 490 and 630 nm) |
| 10002699 | ChroMate® ELISA Reader (Awareness Technology, Inc.) | ChroMate® 4300 |
Zearalenone Cleanup Columns
ZON columns range from simple one-step cleanup with MycoSep® and MultiSep® technology to immunoaffinity cleanup. These cleanup columns, some of which part of AOAC and CEN methods, ensure fast and reliable purification of your samples.
MycoSep® & MultiSep®
Cleanup Columns
MycoSep® and MultiSep® columns contain packing materials made of adsorbent mixes specially designed for all food and feed commodities, for a fast cleanup within 30 seconds.
Click on the item numbers below for more information.
| Item No. | Product | Specificity | No. of Columns |
| MycoSep® | |||
|---|---|---|---|
| 10001948 | MycoSep® 112 AflaZon | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| 100019512 | MycoSep® 224 AflaZon | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| 10001953 | MycoSep® 226 AflaZon+ | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| Item No. | Product | Specificity | No. of Columns |
| MultiSep® | |||
|---|---|---|---|
| 10001939 | MultiSep® 224 AflaZon | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| 10001941 | MultiSep® 226 AflaZon+ | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
2AOAC official method
MycoSpin®
Cleanup Columns
Romer Labs MycoSpin® 400 multi-mycotoxin two-step cleanup column in a spin format was developed to be used for most regulated mycotoxins to shorten the time-consuming cleanup process. Multiple mycotoxins can be analyzed simultaneously by LC-MS/MS.
Highly accurate LC-MS/MS results are achieved by combining the MycoSpin® 400 cleanup with Biopure™ 13C isotope labeled standards.
Click on the item number below for more information.
| Item No. | Product | Specificity | No. of Columns |
| MycoSpin® | |||
|---|---|---|---|
| 10001961 | MycoSpin® 400 Multitoxin | Aflatoxin B1, B2, G1 & G2, Trichothecenes Type A & B, Zearalenone, Ochratoxin A, Fumonisin B1, B2 & B3 | 25 |
StarLineTM
Immunoaffinity Columns
StarLine™ immunoaffinity columns are designed for simple and reliable cleanup before detection of zearalenone in a broad range of different commodities. Those IAC can be used for the purification as well as concentration of mycotoxins prior to analysis by various techniques, such as HPLC, GC-MS, LC-MS, ELISA or direct fluorometry.
Click on the item number below for more information.
| Item No. | Specificity | Format | No. of Columns |
| ZearaStar™ R | |||
|---|---|---|---|
| 10001973 | Zearalenone | 3 mL | 25 |
StarLine™
Immunoaffinity Columns
MultiStar™ immunoaffinity columns belong to the StarLine™ portfolio of immunoaffinity column (IAC). These columns are designed for the reliable clean-up of samples to simplify the detection of several mycotoxins at once in a broad range of different commodities.
MultiStar™ immunoaffinity columns combine the high purification potential of an immunoaffinity column with the convenience and power of multi-mycotoxin analysis, allowing for all regulated mycotoxins to be determined simultaneously in a single run.
| Item no. | Analytes | No. of columns |
| MultiStar™ - Immunoaffinity Column | ||
|---|---|---|
| 10005407 | Aflatoxins, OTA, ZON, DON, FUM, T2 and HT2 | 25 |
BiopureTM
Reference Materials
Under the brand Biopure™, Romer Labs offers a broad range of zearalenone reference materials. These high quality products are available in liquid “ready-to-use” and crystalline forms, and as single calibrants as well as calibrant mixtures.
The stars of the Biopure™ product line are the fully labeled 13C internal standards for mass spectrometry analysis.
Quality control materials (QCM) are offered for method validation purposes and to control the performance of an analytical method.
Here you can download our complete Biopure Product List.
Analytical Service
Romer Labs operates 3 fully accredited mycotoxin service laboratories on 3 continents – Europe, America and Asia -, completing the extensive portfolio of mycotoxin test kits.
Mycotoxin testing services are offered in Austria, the United States and Singapore for a variety of sample matrices, including grains and other raw materials, and feed and food samples.
Both the ISO 9001 certification and ISO 17025 accreditation guarantee reliable and accurate results. We uphold our standards of accuracy and reliability by participating regularly in official proficiency testing programs.
More information on our analytical service offering can be found here.
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The major species of fungus responsible for producing this mycotoxin is Fusarium graminearum.... more
