Test Kits for the Accurate Detection of Melamine

Melamine, a by-product of the coal industry, is a chemical compound frequently used for the production of plastics, kitchenware, commercial filters, laminates or coatings.

Melamine is very high in nitrogen. This has led to its illegal addition to food and feed for alteration as the high nitrogen content increases the apparent amount of protein in standard measurement procedures.

Consequences of illegal melamine food adulteration

Melamine first became a topic of great discussion in 2007, when veterinary scientists determined it to be the cause of hundreds of pet deaths from pet food contamination.

In September 2008, melamine was found in milk and infant formula, where it had been added to give appearance of higher protein content. The presence of melamine led to kidney stones and renal failure among young children in China. More than 50,000 people became ill, with 4 infant deaths and more than 12,800 hospitalizations.

Fast and highly sensitive melamine analysis

The Romer Labs test kit portfolio covers 2 highly sensitive ELISA methods for the quantitative analysis of melamine in different food and feed matrices.




AgraQuant® test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format.

The AgraQuant® Melamine test kit has been validated for analyzing dried distillers grains, soy bean meal and pet food. The quantitation range is 10 - 250 ppm for wheat gluten samples and 2 - 50 ppm for other commodity matrices.

The AgraQuant® Melamine Sensitive test kit is an extremely sensitive ELISA method which has been specifically developed for the detection of melamine in egg and dairy products as well as other foods. The quantitation range is 0.1 - 5 ppm for milk, yogurt, yogurt drinks and eggs, and 0.5 - 25 ppm for milk powder and biscuit samples.

Both the AgraQuant® Melamine and AgraQuant® Melamine Sensitive test kits come complete with melamine standards, antibody coated microwells, a sample diluent, a wash buffer, an enzyme conjugate, a substrate and a stop solution. 

Click on the item numbers below for more information.

Item No. Quantitation Range/Cutof Limit Of Detection Number Of Wells
AgraQuant® Melamine
10002124 wheat gluten: 10-250 ppm; pet food, soybean & corn germ meal: 2-50 ppm; DDGS: 5-50 ppm 1.5 ppm (1.5 mg/kg) for dried pet food; 0.9 ppm (0.9 mg/kg) for corn germ meal; 0.2 ppm (0.2 mg/kg) for soybean meal; 4.0 ppm (4.0 mg/kg) for DDGS; 6.7 ppm (6.7 mg/kg) for wheat gluten 96
AgraQuant® Melamine Sensitive
10002125 milk, yoghurt, yoghurt drink, egg: 0.1-5 ppm; milk powder, biscuit: 0.5-25 ppm 8 ppb (8 µg/kg) for milk; 150 ppb (150 µg/kg) for milk powder 96
Item No. Product Description
Readers for Result Quantification
10002756 StatFax® ELISA Reader (Awareness Technology, Inc.) Stat Fax® 4700
10002740 800 TS Reader incl. Gen5 SW  (BioTek Instruments Inc.) BioTek® 800 TS Compact Microplate Reader (405, 450, 490 and 630 nm)
10002741 800 TS Reader (BioTek Instruments Inc.) BioTek® 800 TS Compact Microplate Reader(405, 450, 490 and 630 nm)
10002699 ChroMate® ELISA Reader (Awareness Technology, Inc.) ChroMate® 4300


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