AgraQuant® B-AGONISTS

VDR | 10002162 (COKDA0600)
Limited availability

Description

AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. The ?-Agonists test kit can be used for the detection of Salbutamol, Clenbuterol and other ?-Agonists in urine and tissue samples of farm animals using either a solid phase extraction (SPEC) or a liquid-liquid extraction (LLE) method.

Properties

Properties
Storage temperature2°C - 8°C
Quantitation range0.5 - 50.0 ppb
Limit of detection0.28 - 0.73 ppb
Commoditymeat (porcine), urine (bovine), feed (porcine)
Analyte?-Agonists (no cross reactivity to Timolo, Metoprolol, Atenolol, Propranolol, Ractopamine; cross reactivity to Clenbuterol (100%), Salbutamol (100%), Terbutaline (40%)
Number of wells96

Materials supplied

  • 1 microtitre plate (96 wells
  • 12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 6 bottles of ?-Agonists calibrators (0 ng/mL; 0.1 ng/mL; 0.2 ng/mL; 0.5 ng/mL; 1 ng/mL; 5 ng/mL) (1 mL each)
  • 2 bottles of ?-Agonists-HRP conjugate solution (6 mL total)
  • 1 bottle of dilution buffer
  • pH 7.5 (100 mL)
  • 1 bottle of wash buffer (20x concentrate; 20 mL)
  • 1 bottle of TMB substrate solution (20 mL)
  • 1 bottle of stop solution (10 mL)

Materials required but not supplied

  • Protease
  • Methanol
  • Distilled water
  • 1 M HCl
  • Isobutanol
  • 0.2 M acetate buffer
  • C18 cartridges
  • Centrifuge
  • 0.25 M sodium carbonate
  • Pipettes
  • Drying system with N2 gas
  • Microplate reader
  • Timer
  • Vortex mixer
  • pH paper
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