AgraQuant® CHLORAMPHENICOL (CAP) PLUS

VDR | 10002175 (COKDA2500)
Limited availability

Description

AgraQuant® Chloramphenicol Plus is a direct competitive enzyme-linked immunosorbent assay (ELISA) that determines a quantitative level of the presence of Chloramphenicol in food. It is a highly sensitive detection system designed for the quantification of Chloramphenicol in urine, serum, liver, milk, egg and tissue samples.

Properties

Properties
Storage temperature2°C - 8°C
Quantitation range0.3 - 6 ppb
Limit of detection0.02 - 0.33 ppb
Commoditytissue sample, urine, serum, milk (liquid, powder), egg, honey, feed, kidney
AnalyteChloramphenicol
Number of wells96

Materials supplied

  • 1 microtitre plate (96 wells - 12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 5 bottles of chloramphenicol calibrators (0 ng/mL; 0.06 ng/mL; 0.2ng/mL; 0.6 ng/mL; 2 ng/mL) (1 mL each)
  • 2 bottles of CAP-HRP conjugate solution (6 mL total)
  • 1 bottle of dilution buffer pH 7.5 (100 mL)
  • 1 bottle of wash buffer (20x concentrate; 20 mL)
  • 1 bottle of TMB substrate solution (20 mL)
  • 1 bottle of stop solution (10 mL)

Materials required but not supplied

  • Ethyl acetate
  • n-hexane
  • Distilled water
  • Iso-octane/chloroform (2:3)
  • Centrifuge
  • Sample tubes
  • Drying system with N2 gas
  • Pipettes
  • Timer
  • Microplate reader
  • pH paper
  • Vortex mixer
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