AgraQuant® CHLORAMPHENICOL

VDR | 10002166 (COKDA1100)
Limited availability

Description

AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. With this ELISA kit, chloramphenicol can be detected in various matrices using a one-step incubation. Chloramphenicol can be detected in tissue samples following an ethyl acetate extraction method, which increases the sensitivity and specificity of the test. This is followed by a clean-up step involving the use of iso-octane/chloroform.

Properties

Properties
Storage temperature2°C - 8°C
Quantitation range0.05 - 10 ppb
Limit of detection0.03 - 0.81ppb
Commodityurine, serum, milk (liquid, powder), tissue samples (chicken, shrimp), egg, honey
AnalyteChloramphenicol (no cross reactivity to Thiamfenicol, Florfenicol; cross reactivity to Chloramphenicol (100%), Chloramphenicol-glucuronide (65%)
Number of wells96

Materials supplied

  • 1 microtitre plate (96 wells
  • 12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 6 bottles of chloramphenicol calibrators (0 ng/mL; 0.05 ng/mL; 0.1 ng/mL; 0.2 ng/mL; 0.5 ng/mL; 2 ng/mL) (1 mL each)
  • 2 bottles of CAP-HRP conjugate solution (6 mL total)
  • 1 bottle of dilution buffer pH 7.5 (100 mL)
  • 1 bottle of wash buffer (20x concentrate; 20 mL)
  • 1 bottle of TMB substrate solution (20 mL)
  • 1 bottle of stop solution (10 mL)

Materials required but not supplied

  • Ethyl acetate
  • n-hexane
  • Distilled water
  • Iso-octane/chloroform (2:3)
  • Centrifuge
  • Sample tubes
  • Drying system with N2 gas
  • Pipettes
  • Timer
  • Microplate reader
  • pH paper
  • Vortex mixer
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