AgraQuant® CIPROFLOXACIN

VDR | 10002165 (COKDA0900)
Limited availability

Description

AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. In this ELISA kit, ciprofloxacin can be detected both in tissue samples as well as in urine samples using methonolic solution.

Properties

Properties
Storage temperature2°C - 8°C
Quantitation range3 - 360 ppb
Limit of detection0.73 - 2.9 ppb
Commodityshrimp, chicken, urine
AnalyteCiprofloxacin (no cross reactivity to Difloxacin, cross reactivity to: Ciprofloxacin (100%), Enrofloxacin (> 100%), Danofloxacin (> 100%), Norfloxacin (33%), Ofloxacin (24%), Enoxacin (7%), Flumequine (6%), Oxolinic Acid (5%), Marbofloxacin (3%)
Number of wells96
Molecular weight (g/mol)trip

Materials supplied

  • 1 microtitre plate (96 wells - 12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 5 bottles of ciprofloxacin calibrators (0 ng/mL; 0.3 ng/mL; 1 ng/mL; 3 ng/mL; 9 ng/mL) (1 mL each)
  • 1 bottle of ciprofloxacin-HRP conjugate solution (4.5 mL)
  • 1 bottle of ciprofloxacin-antibody solution (4.5 mL)
  • 1 bottle of dilution buffer
  • pH 7.5 (100 mL)
  • 1 bottle of wash buffer (20x concentrate; 20 mL)
  • 1 bottle of TMB substrate solution (20 mL)
  • 1 bottle of stop solution (10 mL)

Materials required but not supplied

  • Methanol
  • Sample tubes
  • Pipettes
  • Drying system with N2 gas
  • Centrifuge
  • Microplate reader
  • Timer
  • Vortex mixer
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