AgraQuant® Dexamethasone

VDR | 10002164 (COKDA0800)
Limited availability

Description

AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. In this ELISA kit, ciprofloxacin can be detected both in tissue samples as well as in urine samples using methonolic solution.

Properties

Properties
Storage temperature2°C - 8°C
Quantitation rangeLiver 0.6 – 20 ppb, Muscle 0.4 – 10 ppb, Urine 5 – 25 ppb, Feed 0.15 – 5 ppb, Milk powder 0.4 – 3 ppb, Milk 0.15 – 2.5 ppb
Limit of detectionLiver 0.22 ppb, Muscle 0.16 ppb, Urine 1.00 ppb, Feed 0.05 ppb, Milk powder 0.11 ppb, Milk 0.08 ppb
Limit of quantitationLiver 0.60 ppb, Muscle 0.40 ppb, Urine 5.00 ppb, Feed 0.15 ppb, Milk powder 0.40 ppb, Milk 0.15 ppb
CommodityLiver, Muscle, Urine , Feed , Milk powder, Milk
AnalyteDexamethasone
Number of wells96

Materials supplied

  • 1 microtitre plate (96 wells - 12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 6 bottles of dexamethasone calibrators (0 ng/mL; 0.05 ng/mL; 0.15 ng/mL; 0.5 ng/mL; 1.5 ng/mL and 5 ng/mL) (1 mL each)
  • 1 bottle of dexamethasone-HRP conjugate solution (4.5 mL)
  • 1 bottle of dexamethasone antibody solution (4.5 mL)
  • 1 bottle of dilution buffer - pH 7.5 (100 mL)
  • 1 bottle of wash buffer (20x concentrate; 20 mL)
  • 1 bottle of TMB substrate solution (20 mL)
  • 1 bottle of stop solution (10 mL)

Materials required but not supplied

  • ethyl acetate
  • distilled water
  • n-hexane
  • centrifuge
  • pH paper
  • pipettes
  • drying system with N2 gas
  • microplate reader
  • timer
  • vortex mixer
  • sample tube
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