AgraQuant® NITROFURAN (AOZ) PLUS

VDR | 10002173 (COKDA2300)
Limited availability

Description

AgraQuant® Nitrofurans (AOZ) Plus is a direct competitive enzyme-linked immunosorbent assay (ELISA) that determines a quantitative level of the presence of AOZ (3-amino-5-morpholino-2-oxazolidone) in food. It represents a highly sensitive detection system and is designed for the quantification of AOZ in tissue samples.

Properties

Properties
Storage temperature2°C - 8°C
Quantitation range0.1 - 3 ppb
Limit of detection0.06 - 0.35 ppb
Commodityshrimp, chicken, fish, urine, honey
AnalyteNitrofuran (AOZ) (no cross reactivity to Nitrofurans (AHD), Nitrofurans (AMOZ), Nitrofurans (SEM) & Nitrofurans (DNSH))
Number of wells96

Materials supplied

  • 1 microtitre plate (96 wells
  • 12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 5 bottles of Nitrofuran (AOZ) calibrators (0 ng/mL; 0.05 ng/mL; 0.15 ng/mL; 0.5 ng/mL; 1.5 ng/mL) (1 mL each)
  • 2 bottles of AOZ-HRP conjugate solution (6 mL total)
  • 1 bottle of dilution buffer
  • pH 7.5 (100 mL)
  • 1 bottle of wash buffer (20x concentrate; 20 mL)
  • 1 bottle of TMB substrate solution (20 mL)
  • 1 bottle of stop solution (10 mL)

Materials required but not supplied

  • Ethyl acetate
  • Dimethylsulfoxide (DMSO)
  • 2-Nitrobenzaldehyde
  • 1 M HCl
  • 1 M NaOH
  • n-hexane or n-heptane
  • 0.1 M K2HPO4
  • Centrifuge
  • Drying system with N2 gas
  • Pipettes
  • Timer
  • Microplate reader
  • Sample tube
  • Vortex mixer
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