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AgraQuant® NITROFURAN (AOZ)

VDR | 10002158 (COKDA0300)
Limited availability

Description

AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. AOZ in the tissue samples is first derivatised with 2-nitrobenzoic aldehyde, and then by an ethyl acetate extraction method, which increases the sensitivity and specificity of the test. This is followed by a clean-up step involving the use of iso-octane/chloroform.

Properties

Properties
Storage temperature2°C - 8°C
Quantitation range0.1 – 3 ppb
Limit of detection0.1 ppb
Number of wells96

Materials supplied

  • 1 microtitre plate (96 wells
  • 12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 6 bottles of AOZ calibrators (0 ng/mL; 0.05 ng/mL; 0.1 ng/mL; 0.2 ng/mL; 0.5 ng/mL; 1.5 ng/mL) (1 mL each)
  • 1 bottle of AOZ-HRP conjugate solution (4.5 mL)
  • 1 bottle of AOZ antibody solution (4.5 mL)
  • 1 bottle of dilution buffer pH 7.5 (100 mL)
  • 1 bottle of wash buffer (20x concentrate; 20 mL)
  • 1 bottle of TMB substrate solution (20 mL)
  • 1 bottle of stop solution (10 mL)

Materials required but not supplied

  • Ethyl acetate
  • Distilled water
  • n-hexane/n-heptane
  • 2-Nitrobenzaldehyde
  • DMSO
  • HCl
  • Incubator
  • K2HPO4
  • NaOH
  • Centrifuge
  • Drying system with N2 gas
  • Pipettes
  • Timer
  • Microplate reader
  • Sample tube
  • Vortex mixer
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