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AgraQuant® QUINOLONE

VDR | 10002167 (COKDA1200)
Limited availability

Description

AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. The quinolones are a family of synthetic, broad-spectrum antibiotics. The majority of quinolones belong to the subgroup of fluoroquinolones which have a fluoro-group attached at the central ring system. The fluoroquinolones belong to the second generation quinolones. Some of these are admitted as antibiotics in veterinary medicine especially for cattle, pigs and chicken. The usage of fluoroquinolones has increased in the past years because large amounts of fluoroquinolones were applied to prevent infectious diseases especially in chicken, swine and fish/shrimp farming.

Properties

Properties
Storage temperature2°C - 8°C
Quantitation range2 - 300 ppb
Limit of detection1.14 - 1.87 ppb
Number of wells96

Materials supplied

  • 1 microtitre plate (96 wells
  • 12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 6 bottles of quinolones calibrators (0 ng/mL; 0.1 ng/mL; 0.3 ng/mL; 1 ng/mL; 5 ng/mL; 15 ng/mL) (1 mL each)
  • 2 bottles of quinolones-HRP conjugate solution (6 mL total)
  • 1 bottle of dilution buffer
  • pH 7.5 (100 mL)
  • 1 bottle of wash buffer (20x concentrate; 20 mL)
  • 1 bottle of TMB substrate solution (20 mL)
  • 1 bottle of stop solution (10 mL)

Materials required but not supplied

  • Methanol
  • Drying system with N2 gas
  • Centrifuge
  • Sample tubes
  • Timer
  • Pipettes
  • Vortex mixer
  • Microplate reader
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