AgraQuant® RACTOPAMINE

VDR | 10002161 (COKDA0500)
Limited availability

Description

AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. This ELISA kit can be used for the detection of ractopamine and its metabolites in urine and tissue samples using either a solid phase extraction (SPEC) or a liquid-liquid extraction (LLE) method.

Properties

Properties
Storage temperature2°C - 8°C
Quantitation range0.30 - 20.0 ppb
Limit of detection0.21 - 3.53 ppb
Commoditymeat, urine, feed
AnalyteRactopamine (no cross reactivity to salbutamol, terbutaline, clenbuterol)
Number of wells96

Materials supplied

  • 1 microtitre plate (96 wells
  • 12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 6 bottles of ractopamine calibrators (0 ng/mL; 0.06 ng/mL; 0.1 ng/mL; 0.3 ng/mL; 0.9 ng/mL; 2 ng/mL) (1 mL each)
  • 2 bottles of ractopamine-HRP conjugate solution (6 mL total)
  • 1 bottle of dilution buffer pH 7.5 (100 mL)
  • 1 bottle of wash buffer (20x concentrate; 20 mL)
  • 1 bottle of TMB substrate solution (20 mL)
  • 1 bottle of stop solution (10 mL)

Materials required but not supplied

  • Protease
  • Methanol
  • Helix pomatia enzyme
  • Distilled water
  • Isobutanol
  • 1 M HCl
  • C18 cartridges
  • 0.2 M acetate buffer
  • 0.25 M sodium carbonate
  • Centrifuge
  • Drying system with N2 gas
  • Pipettes
  • Timer
  • Microplate reader
  • pH paper
  • Vortex mixer
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