AgraQuant® B-AGONISTS
VDR | 10002162 (COKDA0600)
Limited availability
Description
AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. The B-Agonists test kit can be used for the detection of Salbutamol, Clenbuterol and other B-Agonists in urine and tissue samples of farm animals using either a solid phase extraction (SPEC) or a liquid-liquid extraction (LLE) method.
Properties
| Storage temperature | 2°C - 8°C |
|---|---|
| Quantitation range | 0.5 - 50.0 ppb |
| Limit of detection | 0.28 - 0.73 ppb |
| Commodity | meat (porcine), urine (bovine), feed (porcine) |
| Analyte | B-Agonists (no cross reactivity to Timolo, Metoprolol, Atenolol, Propranolol, Ractopamine; cross reactivity to Clenbuterol (100%), Salbutamol (100%), Terbutaline (40%) |
| Number of wells | 96 |
Materials supplied
- 1 microtitre plate (96 wells
- 12 breakable strips) sealed in an aluminium zip lock bag
- 1 piece of sealing tape
- 6 bottles of B-Agonists calibrators (0 ng/mL; 0.1 ng/mL; 0.2 ng/mL; 0.5 ng/mL; 1 ng/mL; 5 ng/mL) (1 mL each)
- 2 bottles of B-Agonists-HRP conjugate solution (6 mL total)
- 1 bottle of dilution buffer
- pH 7.5 (100 mL)
- 1 bottle of wash buffer (20x concentrate; 20 mL)
- 1 bottle of TMB substrate solution (20 mL)
- 1 bottle of stop solution (10 mL)
Materials required but not supplied
- Protease
- Methanol
- Distilled water
- 1 M HCl
- Isobutanol
- 0.2 M acetate buffer
- C18 cartridges
- Centrifuge
- 0.25 M sodium carbonate
- Pipettes
- Drying system with N2 gas
- Microplate reader
- Timer
- Vortex mixer
- pH paper
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