AgraQuant® CHLORAMPHENICOL
VDR | 10002166 (COKDA1100)
Limited availability
Description
AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. With this ELISA kit, chloramphenicol can be detected in various matrices using a one-step incubation. Chloramphenicol can be detected in tissue samples following an ethyl acetate extraction method, which increases the sensitivity and specificity of the test. This is followed by a clean-up step involving the use of iso-octane/chloroform.
Properties
| Storage temperature | 2°C - 8°C |
|---|---|
| Quantitation range | 0.05 - 10 ppb |
| Limit of detection | 0.03 - 0.81ppb |
| Commodity | urine, serum, milk (liquid, powder), tissue samples (chicken, shrimp), egg, honey |
| Analyte | Chloramphenicol (no cross reactivity to Thiamfenicol, Florfenicol; cross reactivity to Chloramphenicol (100%), Chloramphenicol-glucuronide (65%) |
| Number of wells | 96 |
Materials supplied
- 1 microtitre plate (96 wells
- 12 breakable strips) sealed in an aluminium zip lock bag
- 1 piece of sealing tape
- 6 bottles of chloramphenicol calibrators (0 ng/mL; 0.05 ng/mL; 0.1 ng/mL; 0.2 ng/mL; 0.5 ng/mL; 2 ng/mL) (1 mL each)
- 2 bottles of CAP-HRP conjugate solution (6 mL total)
- 1 bottle of dilution buffer pH 7.5 (100 mL)
- 1 bottle of wash buffer (20x concentrate; 20 mL)
- 1 bottle of TMB substrate solution (20 mL)
- 1 bottle of stop solution (10 mL)
Materials required but not supplied
- Ethyl acetate
- n-hexane
- Distilled water
- Iso-octane/chloroform (2:3)
- Centrifuge
- Sample tubes
- Drying system with N2 gas
- Pipettes
- Timer
- Microplate reader
- pH paper
- Vortex mixer
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