AgraQuant® NITROFURAN (AHD)
VDR | 10002174 (COKDA2400)
Limited availability
Description
AgraQuant® Nitrofuran (AHD) is a direct competitive enzyme-linked immunosorbent assay (ELISA) that determines a quantitative level of the presence of AHD (1-aminohydantoin) in food. It is a highly sensitive detection system designed for the quantification of AHD in urine, honey and meat (muscle/tissue) samples.
Properties
PLM Item Name | AGRAQUANT NITROFURAN (AHD) ASSAY (96 WELLS) |
---|---|
Storage temperature | 2°C - 8°C |
Quantitation range | 0.20 - 16 ppb |
Limit of detection | 0.07 - 0.34 ppb |
Analyte | Nitrofurans (AHD) (no cross reactivity to Nitrofurans (AMOZ), Nitrofurans (AOZ), Nitrofurans (SEM) & Nitrofurans (DNSH)) |
Number of wells | 96 |
Materials supplied
- 1 microtitre plate (96 wells
- 12 breakable strips) sealed in an aluminium zip lock bag
- 1 piece of sealing tape
- 5 bottles of Nitrofuran (AHD) calibrators (0 ng/mL; 0.1 ng/mL; 0.4 ng/mL; 2 ng/mL; 8 ng/mL) (1 mL each)
- 2 bottles of AHD-HRP conjugate solution (6 mL total)
- 1 bottle of dilution buffer pH 7.5 (100 mL)
- 1 bottle of wash buffer (20x concentrate; 20 mL)
- 1 bottle of TMB substrate solution (20 mL)
- 1 bottle of stop solution (10 mL)
Materials required but not supplied
- Ethyl acetate
- Dimethylsulfoxide (DMSO)
- 2-Nitrobenzaldehyde
- 1 M HCl
- 1 M NaOH
- n-hexane or n-heptane
- 0.1 M K2HPO4
- Centrifuge
- Drying system with N2 gas
- Pipettes
- Timer
- Microplate reader
- Sample tube
- Vortex mixer
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