AgraQuant® NITROFURAN (AOZ) PLUS
VDR | 10002173 (COKDA2300)
Limited availability
Description
AgraQuant® Nitrofurans (AOZ) Plus is a direct competitive enzyme-linked immunosorbent assay (ELISA) that determines a quantitative level of the presence of AOZ (3-amino-5-morpholino-2-oxazolidone) in food. It represents a highly sensitive detection system and is designed for the quantification of AOZ in tissue samples.
Properties
| PLM Item Name | AGRAQUANT NITROFURAN (AOZ) PLUS ASSAY (96 WELLS) |
|---|---|
| Storage temperature | 2°C - 8°C |
| Quantitation range | 0.1 - 3 ppb |
| Limit of detection | 0.06 - 0.35 ppb |
| Analyte | Nitrofuran (AOZ) (no cross reactivity to Nitrofurans (AHD), Nitrofurans (AMOZ), Nitrofurans (SEM) & Nitrofurans (DNSH)) |
| Number of wells | 96 |
Materials supplied
- 1 microtitre plate (96 wells
- 12 breakable strips) sealed in an aluminium zip lock bag
- 1 piece of sealing tape
- 5 bottles of Nitrofuran (AOZ) calibrators (0 ng/mL; 0.05 ng/mL; 0.15 ng/mL; 0.5 ng/mL; 1.5 ng/mL) (1 mL each)
- 2 bottles of AOZ-HRP conjugate solution (6 mL total)
- 1 bottle of dilution buffer
- pH 7.5 (100 mL)
- 1 bottle of wash buffer (20x concentrate; 20 mL)
- 1 bottle of TMB substrate solution (20 mL)
- 1 bottle of stop solution (10 mL)
Materials required but not supplied
- Ethyl acetate
- Dimethylsulfoxide (DMSO)
- 2-Nitrobenzaldehyde
- 1 M HCl
- 1 M NaOH
- n-hexane or n-heptane
- 0.1 M K2HPO4
- Centrifuge
- Drying system with N2 gas
- Pipettes
- Timer
- Microplate reader
- Sample tube
- Vortex mixer
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