AgraQuant® NITROFURAN (AOZ)
VDR | 10002158 (COKDA0300)
Limited availability
Description
AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. AOZ in the tissue samples is first derivatised with 2-nitrobenzoic aldehyde, and then by an ethyl acetate extraction method, which increases the sensitivity and specificity of the test. This is followed by a clean-up step involving the use of iso-octane/chloroform.
Properties
| PLM Item Name | AGRAQUANT NITROFURAN (AOZ) (96 WELLS) |
|---|---|
| Storage temperature | 2°C - 8°C |
| Quantitation range | 0.1 – 3 ppb |
| Limit of detection | 0.1 ppb |
| Analyte | AOZ (no cross reactivity to AMOZ, AHD, SEM) |
| Number of wells | 96 |
Materials supplied
- 1 microtitre plate (96 wells
- 12 breakable strips) sealed in an aluminium zip lock bag
- 1 piece of sealing tape
- 6 bottles of AOZ calibrators (0 ng/mL; 0.05 ng/mL; 0.1 ng/mL; 0.2 ng/mL; 0.5 ng/mL; 1.5 ng/mL) (1 mL each)
- 1 bottle of AOZ-HRP conjugate solution (4.5 mL)
- 1 bottle of AOZ antibody solution (4.5 mL)
- 1 bottle of dilution buffer pH 7.5 (100 mL)
- 1 bottle of wash buffer (20x concentrate; 20 mL)
- 1 bottle of TMB substrate solution (20 mL)
- 1 bottle of stop solution (10 mL)
Materials required but not supplied
- Ethyl acetate
- Distilled water
- n-hexane/n-heptane
- 2-Nitrobenzaldehyde
- DMSO
- HCl
- Incubator
- K2HPO4
- NaOH
- Centrifuge
- Drying system with N2 gas
- Pipettes
- Timer
- Microplate reader
- Sample tube
- Vortex mixer
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