AgraQuant® RACTOPAMINE
VDR | 10002161 (COKDA0500)
Limited availability
Description
AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. This ELISA kit can be used for the detection of ractopamine and its metabolites in urine and tissue samples using either a solid phase extraction (SPEC) or a liquid-liquid extraction (LLE) method.
Properties
PLM Item Name | AGRAQUANT RACTOPAMINE (96 WELLS) |
---|---|
Storage temperature | 2°C - 8°C |
Quantitation range | 0.30 - 20.0 ppb |
Limit of detection | 0.21 - 3.53 ppb |
Analyte | Ractopamine (no cross reactivity to salbutamol, terbutaline, clenbuterol) |
Number of wells | 96 |
Materials supplied
- 1 microtitre plate (96 wells
- 12 breakable strips) sealed in an aluminium zip lock bag
- 1 piece of sealing tape
- 6 bottles of ractopamine calibrators (0 ng/mL; 0.06 ng/mL; 0.1 ng/mL; 0.3 ng/mL; 0.9 ng/mL; 2 ng/mL) (1 mL each)
- 2 bottles of ractopamine-HRP conjugate solution (6 mL total)
- 1 bottle of dilution buffer pH 7.5 (100 mL)
- 1 bottle of wash buffer (20x concentrate; 20 mL)
- 1 bottle of TMB substrate solution (20 mL)
- 1 bottle of stop solution (10 mL)
Materials required but not supplied
- Protease
- Methanol
- Helix pomatia enzyme
- Distilled water
- Isobutanol
- 1 M HCl
- C18 cartridges
- 0.2 M acetate buffer
- 0.25 M sodium carbonate
- Centrifuge
- Drying system with N2 gas
- Pipettes
- Timer
- Microplate reader
- pH paper
- Vortex mixer
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