10002156
AgraQuant® NITROFURAN (AMOZ)
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AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. AMOZ in the tissue samples is first derivatised with 2-nitrobenzoic aldehyde, and then by an ethyl acetate extraction method, which increases the sensitivity and specificity of the test. This is then followed by a clean-up step involving the use of iso-octane/chloroform.
Rango de cuantificación/Corte | 0.2 – 16.2 ppb |
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Límite de detección | 0.2 ppb |
Materias primas | tissue sample (shrimp, chicken) |
Número de pozos | 96 |
Materials
Materiales suministrados
- 1 microtitre plate (96 wells -12 breakable strips) sealed in an aluminium zip lock bag
- 1 piece of sealing tape
- 6 bottles of AMOZ calibrators (0 ng/mL; 0.1 ng/mL; 0.3 ng/mL; 0.9ng/mL; 2.7 ng/mL; 8.1 ng/mL) (1 mL each)
Materiales requeridos pero no suministrados
- Ethyl acetate
- Distilled water
- n-hexane/n-heptane
- 2-Nitrobenzaldehyde
- DMSO
- HCl
- Incubator
- K2HPO4
- NaOH
- Centrifuge
- Drying system with N2 gas
- Pipettes
- Timer
- Microplate reader
- Sample tube
- Vortex mixer