10002175
AgraQuant® CHLORAMPHENICOL (CAP) PLUS
Limited availability
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Contact usDescription & Properties
AgraQuant® Chloramphenicol Plus is a direct competitive enzyme-linked immunosorbent assay (ELISA) that determines a quantitative level of the presence of Chloramphenicol in food. It is a highly sensitive detection system designed for the quantification of Chloramphenicol in urine, serum, liver, milk, egg and tissue samples.
| Quantitation range | 0.3 - 6 ppb |
|---|---|
| Limit of detection | 0.02 - 0.33 ppb |
| Commodity | tissue sample, urine, serum, milk (liquid, powder), egg, honey, feed, kidney |
| Number of wells | 96 |
Materials
Materials supplied
- 1 microtitre plate (96 wells - 12 breakable strips) sealed in an aluminium zip lock bag
- 1 piece of sealing tape
- 5 bottles of chloramphenicol calibrators (0 ng/mL; 0.06 ng/mL; 0.2ng/mL; 0.6 ng/mL; 2 ng/mL) (1 mL each)
- 2 bottles of CAP-HRP conjugate solution (6 mL total)
- 1 bottle of dilution buffer pH 7.5 (100 mL)
- 1 bottle of wash buffer (20x concentrate; 20 mL)
- 1 bottle of TMB substrate solution (20 mL)
- 1 bottle of stop solution (10 mL)
Materials required but not supplied
- Ethyl acetate
- n-hexane
- Distilled water
- Iso-octane/chloroform (2:3)
- Centrifuge
- Sample tubes
- Drying system with N2 gas
- Pipettes
- Timer
- Microplate reader
- pH paper
- Vortex mixer
