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Description & Properties

AgraQuant® drug residue test kits are accurate and reliable direct competitive enzyme-linked immunosorbent assays (ELISA) which operate on the basis of competition between the drug/metabolite in the sample and the drug enzyme conjugate for a limited number of specific antibody binding sites. AMOZ in the tissue samples is first derivatised with 2-nitrobenzoic aldehyde, and then by an ethyl acetate extraction method, which increases the sensitivity and specificity of the test. This is then followed by a clean-up step involving the use of iso-octane/chloroform.
Quantitation range0.2 – 16.2 ppb
Limit of detection0.2 ppb
Commoditytissue sample (shrimp, chicken)
Number of wells96


Materials supplied

  • 1 microtitre plate (96 wells -12 breakable strips) sealed in an aluminium zip lock bag
  • 1 piece of sealing tape
  • 6 bottles of AMOZ calibrators (0 ng/mL; 0.1 ng/mL; 0.3 ng/mL; 0.9ng/mL; 2.7 ng/mL; 8.1 ng/mL) (1 mL each)

Materials required but not supplied

  • Ethyl acetate
  • Distilled water
  • n-hexane/n-heptane
  • 2-Nitrobenzaldehyde
  • DMSO
  • HCl
  • Incubator
  • K2HPO4
  • NaOH
  • Centrifuge
  • Drying system with N2 gas
  • Pipettes
  • Timer
  • Microplate reader
  • Sample tube
  • Vortex mixer

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