Gluten Test Kits for Lab and On-Site Detection
Gluten is a fraction of cereal proteins that can not only trigger allergic reactions, but also cause an immune response in people suffering from celiac disease. The only treatment in both cases is a life-long gluten-free diet.
It is the responsibility of food producers to protect consumers from the consequences of accidental gluten intake. According to many national authorities and Codex Alimentarius standard 118-1979, the level of gluten in food must not exceed 20 mg/kg (20 ppm) for products to be labeled gluten-free.
Highly sensitive gluten detection
The Romer Labs gluten test kit portfolio covers ELISAs and LFDs for the quantitative and qualitative analysis of gluten in environmental samples, rinse waters and finished food products.
Certified and AOAC approved gluten test kits
The AgraQuant® Gluten G12 ELISA test kit is an 
AACC International Approved method (AACCI 38-52.01) and an AOAC-approved Official Method of Analysis (OMA 2014.03).
The AgraStrip® Gluten G12 LFD test kit holds the certification of the AOAC Research Institute for Performance Tested methods (Certificate No. 061403).
Highly specific next generation antibody - G12
Besides the polyclonal gluten antibody used in its traditional test kits, Romer Labs offers methods that employ a next generation antibody, the G12. This monoclonal antibody was raised against an Alpha-2-gliadin fragment that is 33 amino acids long and a principal contributor to gluten immunotoxicity. The 33-mer is highly resistant to digestive degradation and is therefore well suited as an analytical marker.
The G12 antibody targets a toxic peptide of gluten that triggers the autoimmune reaction in celiac patients, making it more relevant for food safety.
AgraStrip®
Lateral Flow Devices
The AgraStrip® Gluten and AgraStrip® Gluten G12 test kits are ready-to-use lateral flow devices (LFD) for on-site testing. These immunological tests allow a rapid analysis of a wide range of food samples within only 11 minutes (including extraction).
AgraStrip® LFDs represent a fast and simple qualitative strip test method in which specific antibodies labeled with colloidal gold are used for visual color development at a test and control zone.
The test kits come complete with strips, antibody coated incubation vials, extraction tubes, an extraction buffer, surface swabs and additional accessories.
Click on the item numbers below for more information.
| Item No. | No. of Tests |
| AgraStrip® Gluten G12 TM | |
|---|---|
| 10001995* | 10 |
| Item No. | No. of Tests |
| AgraStrip® Gluten | |
|---|---|
| 10002000 | 10 |
* performance tested by AOAC: AOAC-RI Approval 061403
AgraQuant®
ELISA Tests
AgraQuant® Gluten/ Gluten G12 test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Results are measured with a specific ELISA reader, such as the StatFax® or the BioTek® reader.
These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with assay times of as low as 60 minutes for up to 38 samples (86 samples for AQ Gluten G12).
AgraQuant® Gluten/ Gluten G12 test kits are sandwich ELISAs in a ready-to-use format to reduce handling errors and come with a set of 5 standards, an antibody coated plate, an extraction solution, a wash buffer, a conjugate, a substrate and a stop solution. The AgraQuant® Gluten G12 additionally contains a diluent buffer and a sachet of fish gelatin.
Click on the item numbers below for more information.
| Item No. | Quantitation Range | Limit of Detection | No. of Wells |
| AgraQuant® Gluten G12 TM | |||
|---|---|---|---|
| 10001994* | 4 - 200 ppm | 2 ppm | 96 |
| Item No. | Quantitation Range | Limit of Detection | No. of Wells |
| AgraQuant® Gluten G12 TM Extraction Buffer | |||
|---|---|---|---|
-
| - | - | |
| Item No. | Quantitation Range | Limit of Detection | No. of Wells |
| AgraQuant® Gluten | |||
|---|---|---|---|
| 10002002 | 4 - 120 ppm | 0.6 ppm | 48 |
* AOAC Official Method of Analysis 2014.03 & AACC International Method 38-52.01
| Item No. | Product | Description |
| Readers for Result Quantification | ||
|---|---|---|
| 10002756 | StatFax® ELISA Reader (Awareness Technology, Inc.) | Stat Fax® 4700 |
| 10002740 | 800 TS Reader incl. Gen5 SW (BioTek Instruments Inc.) | BioTek® 800 TS Compact Microplate Reader (405, 450, 490 and 630 nm) |
| 10002699 | ChroMate® ELISA Reader (Awareness Technology, Inc.) | ChroMate® 4300 |
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