Gluten Test Kits for Lab and On-Site Detection

Gluten is a fraction of cereal proteins that can not only trigger allergic reactions, but also cause an immune response in people suffering from celiac disease. The only treatment in both cases is a life-long gluten-free diet.

It is the responsibility of food producers to protect consumers from the consequences of accidental gluten intake. According to many national authorities and Opens external link in new windowCodex Alimentarius standard 118-1797, the level of gluten in food must not exceed 20 mg/kg (20 ppm) for products to be labeled gluten-free.

Highly sensitive gluten detection

The Romer Labs gluten test kit portfolio covers ELISAs and LFDs for the quantitative and qualitative analysis of gluten in environmental samples, rinse waters and finished food products.

Certified and AOAC approved gluten test kits

The AgraQuant® Gluten G12 ELISA test kit is an Opens external link in new windowAACC International Approved method (AACCI 38-52.01) and an Opens external link in new windowAOAC-approved Official Method of Analysis (OMA 2014.03).

The AgraStrip® Gluten G12 LFD test kit holds the certification of the AOAC Research Institute for Performance Tested methods (Opens external link in new windowCertificate No. 061403).

Highly specific next generation antibody - G12

Besides the polyclonal gluten antibody used in its traditional test kits, Romer Labs offers methods that employ a next generation antibody, the G12. This monoclonal antibody was raised against an Alpha-2-gliadin fragment that is 33 amino acids long and a principal contributor to gluten immunotoxicity. The 33-mer is highly resistant to digestive degradation and is therefore well suited as an analytical marker. 

The G12 antibody targets a toxic peptide of gluten that triggers the autoimmune reaction in celiac patients, making it more relevant for food safety.

AgraStrip

AgraStrip®

Lateral Flow Devices

The AgraStrip® Gluten and AgraStrip® Gluten G12 test kits are ready-to-use lateral flow devices (LFD) for on-site testing. These immunological tests allow a rapid analysis of a wide range of food samples within only 11 minutes (including extraction).

AgraStrip® LFDs represent a fast and simple qualitative strip test method in which specific antibodies labeled with colloidal gold are used for visual color development at a test and control zone.

The test kits come complete with strips, antibody coated incubation vials, extraction tubes, an extraction buffer, surface swabs and additional accessories.

Click on the item numbers below for more information.

Item No.No. of Tests
AgraStrip® Gluten G12 TM
COKAL0200AS*10
Item No.No. of Tests
AgraStrip® Gluten
COKAL0210AS10

  * performance tested by AOAC: AOAC-RI Approval 061403

AgraQuant

AgraQuant®

ELISA Tests

AgraQuant® Gluten/ Gluten G12 test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Results are measured with a specific ELISA reader, such as the StatFax® or the BioTek® reader.

These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with assay times of as low as 60 minutes for up to 38 samples (86 samples for AQ Gluten G12).

AgraQuant® Gluten/ Gluten G12 test kits are sandwich ELISAs in a ready-to-use format to reduce handling errors and come with a set of 5 standards, an antibody coated plate, an extraction solution, a wash buffer, a conjugate, a substrate and a stop solution. The AgraQuant® Gluten G12 additionally contains a diluent buffer and a sachet of fish gelatin.

Click on the item numbers below for more information.

Item No.Quantitation RangeLimit of DetectionNo. of Wells
AgraQuant® Gluten G12 TM
COKAL0200*4 - 200 ppm2 ppm96
Item No.Quantitation RangeLimit of DetectionNo. of Wells
AgraQuant® Gluten G12 TM Extraction Buffer
COKAL0206---
Item No.Quantitation RangeLimit of DetectionNo. of Wells
AgraQuant® Gluten
COKAL02484 - 120 ppm0.6 ppm48

* AOAC Official Method of Analysis 2014.03 & AACC International Method 38-52.01

Item No.ProductDescription
Readers for Result Quantification
EQOLE4700StatFax® ELISA Reader (Awareness Technology, Inc.)Stat Fax® 4700
EQOLE1495ELx800 UV Reader (BioTek Instruments Inc.)BioTek® ELx800 UV Absorbance Microplate Reader
EQOLE1408ChroMate® ELISA Reader (Awareness Technology, Inc.)ChroMate® 4300

 

 

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