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AgraQuant® Gluten G12®

Allergens Gluten ELISA Quantitative


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Description & Properties

The AgraQuant® Gluten G12® is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative analysis of gluten in a variety of foods. Samples can vary from raw to processed foods, from environmental swabs to rinse water. The G12® antibody targets a 33-mer peptide which is resistant to enzymatic digestion and heat denatiuration, and is the fragment of the gliadin protein to which celiac disease sufferers react, making it a reliable analytical marker. This product is intended for laboratory use.
Quantitation range4 - 200 ppm gluten
Limit of detection2 ppm gluten
Limit of quantitation4 ppm gluten
Storage Temperature2°C - 8°C
Result typeQuantitative
Product/EnvironmentEnvironmental Monitoring, Product Testing
ApprovalsAACC international method 38-52.01, AOAC official method analysis (OMA) 2014.03
Number of wells96


Materials supplied

  • 96 antibody-coated microwells (12 eight-well strips) in a microwell holder sealed in a foil pouch
  • 5 ready-to-use standards (0; 4; 20; 80 and 200 ppm)
  • 1 bottle of ready-to-use extraction solution
  • 1 bottle of concentrated (5X) diluent buffer
  • 1 bottle of concentrated (10X) wash buffer
  • 1 green-capped bottle of conjugate solution
  • 1 blue-capped bottle of substrate solution
  • 1 red-capped bottle of stop solution
  • 1 sachet of 10 g fish gelatin

Materials required but not supplied

  • Blender
  • analytical balance
  • 8-channel pipette
  • wash bottle
  • microwells/plate reader


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