DON Testing Solutions for Lab and On-Site Deoxynivalenol Detection

Also known as vomitoxin, deoxynivalenol (DON) is a potent fungal toxin to affect crops grown in cool and moist conditions.

Deoxynivalenol is a known immunosuppressant and may cause kidney problems. Consuming DON-contaminated grain can cause vomiting in humans and animals. Deoxynivalenol is formed by the Fusarium species and mainly affects corn, wheat, oats and barley. Such Fusarium species are also known to co-produce other toxins, especially zearalenone.

Most countries have established regulations to protect consumers from the harmful effects of deoxynivalenol intake. The European Union has established maximum levels for food and recommendations for feed while the US FDA has established advisory levels.



Lateral Flow Devices

The AgraStrip® test kits are ready-to-use lateral flow devices (LFD) for on-site testing. These allow a rapid analysis of a wide range of food and feed samples with an assay time as low as 3 minutes. 

The test kits are available in a qualitative or quantitative format. While qualitative LFDs can be read visually, quantitative tests are used with the AgraVision™ and AgraVision™ Pro readers to provide objective results and secure a consistent results documentation

Click on the item numbers below for more information.

Item No. Quantitation Range Limit of Detection No. of Tests
AgraStrip® Pro Deoxynivalenol WATEX®
10006320 0 – 44 ppm 0.1 ppm) 40
AgraStrip® WATEX® Deoxynivalenol
100021504 (10g extraction) 0 - 30 000 ppb 150 ppb (wheat)
100 ppb (corn)
100021494 (50g extraction) 0 - 30 000 ppb 150 ppb (wheat)
100 ppb (corn)

4AgraVisionTM Reader

Item No. Product
Readers for Result Quantification
10006324 AgraVision™ Pro Reader
10006483 AgraVision™ Pro Printer
10006484 AgraVision™ Pro Scanner
10002418 AgraVisionTM with Printer
10002421 AgraVisionTM
10002425 AgraVisionTM Set-Up with Reader, Printer, Incubator, Tweezer



AgraQuant® Deoxynivalenol test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented.

These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 20 minutes for up to 42 samples.

Test kits are in a ready-to-use format to reduce handling errors and come with a set of 5 standards, an antibody coated plate, a dilution plate, a conjugate, a substrate and a stop solution.

Click on the item numbers below for more information.

Item No. Quantitation Range Limit of Detection No. of Wells
AgraQuant® Deoxynivalenol
100021062,3 250 - 5000 ppb 200 ppb 96
100021082,3 250 - 5000 ppb 200 ppb 48

2Japanese Department of Agriculture approved
3AOAC RI approved

Item No. Product Description
Readers for Result Quantification
10002756 StatFax® ELISA Reader (Awareness Technology, Inc.) Stat Fax® 4700
10002740 800 TS Reader incl. Gen5 SW  (BioTek Instruments Inc.) BioTek® 800 TS Compact Microplate Reader (405, 450, 490 and 630 nm)
10002741 800 TS Reader (BioTek Instruments Inc.) BioTek® 800 TS Compact Microplate Reader(405, 450, 490 and 630 nm)
10002699 ChroMate® ELISA Reader (Awareness Technology, Inc.) ChroMate® 4300


Deoxynivalenol Cleanup Columns

DON columns range from simple one-step cleanup with MycoSep® and MultiSep® technology to immunoaffinity cleanup. These cleanup columns are part of AOAC official methods and/or official methods of the Japanese Department of Agriculture and ensure a fast and reliable purification of your samples.


MycoSep® & MultiSep®   

Cleanup Columns

MycoSep® and MultiSep® columns contain packing materials made of adsorbent mixes specially designed for all food and feed commodities, for a fast cleanup within 30 seconds.

A complete overview can be found below. Click on the item numbers for more information.

Item No. Product Specificity No. of Columns
100019491,2 MycoSep® 113 Trich

Trichothecenes, Type A & Type B

10001952 MycoSep® 225 Trich Trichothecenes, Type A & Type B 25
100019541,2 MycoSep® 227 Trich+ Trichothecenes, Type A & Type B 25
Item No. Product Specificity No. of Columns
10001938 MultiSep® 216 Trichothecenes, Type A & Type B 25
100019401,2 MultiSep® 225 Trich Trichothecenes, Type A & Type B 25
10001942 MultiSep® 227 Trich+ Trichothecenes, Type A & Type B 25

1AOAC official method
2Japanese official method



Cleanup Columns

Romer Labs MycoSpin® 400 multi-mycotoxin two-step cleanup column in a spin format was developed to be used for most regulated mycotoxins to shorten the time-consuming cleanup process. Multiple mycotoxins can be analyzed simultaneously by LC-MS/MS.

Highly accurate LC-MS/MS results are achieved by combining the MycoSpin® 400 cleanup with Biopure™ 13C isotope labeled standards.

Click on the item number below for more information.

Item No. Product Specificity No. of Columns
10001961 MycoSpin® 400 Multitoxin Aflatoxin B1, B2, G1 & G2, Trichothecenes Type A & B, Zearalenone, Ochratoxin A, Fumonisin B1, B2 & B3 25


Immunoaffinity Columns

StarLine™ immunoaffinity columns (IAC) are designed for simple and reliable cleanup prior to deoxynivalenol detection in a broad range of different commodities. The columns can be used to purify and concentrate the DON level prior to analysis by various techniques, such as HPLC, GC-MS, LC-MS, ELISA or direct fluorometry.

Click on the item number below for more information.

Item No. Specificity Format No. of Columns
10001974 Deoxynivalenol 3 mL 25


Immunoaffinity Columns

MultiStar™ immunoaffinity columns belong to the StarLine™ portfolio of immunoaffinity column (IAC). These columns are designed for the reliable clean-up of samples to simplify the detection of several mycotoxins at once in a broad range of different commodities. 
MultiStar™ immunoaffinity columns combine the high purification potential of an immunoaffinity column with the convenience and power of multi-mycotoxin analysis, allowing for all regulated mycotoxins to be determined simultaneously in a single run. 

Item no. Analytes No. of columns
MultiStar™ - Immunoaffinity Column
10005407 Aflatoxins, OTA, ZON, DON, FUM, T2 and HT2 25


Reference Materials

Under the brand Biopure™, Romer Labs offers a broad range of deoxynivalenol reference materials. These high quality products are available in liquid “ready-to-use” and crystalline forms, and as single calibrants as well as calibrant mixtures.

The stars of the Biopure™ product line are the fully labeled 13C internal standards for the detection via mass spectrometry.

Quality control materials (QCM) are offered for method validation purposes and to control the performance of an analytical method. 

Here you can download our complete Biopure Product List.

Analytical Service


Analytical Service

Romer Labs operates 3 fully accredited mycotoxin service laboratories on 3 continents – Europe, America and Asia -, completing the extensive portfolio of mycotoxin test kits.

Mycotoxin testing services are offered in Austria, the United States and Singapore for a variety of sample matrices, including grains and other raw materials, and feed and food samples.

Both the ISO 9001 certification and ISO 17025 accreditation guarantee reliable and accurate results. We uphold our standards of accuracy and reliability by participating regularly in official proficiency testing programs.

More information on our analytical service offering can be found here.

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