Sample Cleanup Columns for Mycotoxins
Romer Labs offers a wide variety of cleanup columns for mycotoxins. These range from simple one-step cleanup with MycoSep® and MultiSep® technology to immunoaffinity cleanup. These cleanup columns, some of which part of AOAC and CEN methods, ensure a fast and reliable purification of your samples.
Romer Labs provides the following cleanup solutions:
MycoSep®
Cleanup Columns
MycoSep® columns represent one-step cleanup columns containing packing materials made of adsorbent mixes specially designed for all food and feed commodities, for fast cleanup within 30 seconds. Cleanup is possible for all EU regulated mycotoxins.
For cleanup with MycoSep® systems, the column is pushed into a test tube containing the sample extract, forcing it to filter upwards. Interferences adhere to the chemical packaging and the purified extract, containing all the mycotoxins, can be collected from the top of the cleanup column.
A complete overview can be found below. Click on the item numbers for more information.
| Item No. | Product | Specificity | No. of Columns |
| MycoSep® | |||
|---|---|---|---|
| COCMY2112 | MycoSep® 112 AflaZon | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| COCMY21131,2 | MycoSep® 113 Trich | Trichothecenes, Type A & B | 25 |
| COCMY2150 | MycoSep® 150 Ergot | Ergot Alkaloids | 25 |
| COCMY22241 | MycoSep® 224 AflaZon | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| COCMY2225 | MycoSep® 225 Trich | Trichothecenes, Type A & B | 25 |
| COCMY2226 | MycoSep® 226 AflaZon+ | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| COCMY22271,2 | MycoSep® 227 Trich+ | Trichothecenes, Type A & B | 25 |
| COCMY22282 | MycoSep® 228 AflaPat | Aflatoxins B1, B2, G1 & G2, Patulin | 25 |
| COCMY2229 | MycoSep® 229 Ochra | Ochratoxin A | 25 |
| COCMY2230 | MycoSep® 230 Niv | Nivalenol | 25 |
| COCMY2231 | MycoSep® 231 Fum | Fumonisin B1, B2 & B3 | 25 |
| COCMY2240 | MycoSep® 240 Mon | Moniliformin | 25 |
1AOAC official method
2Japanese official method
MultiSep®
Cleanup Columns
MultiSep® columns represent one-step cleanup columns containing packing materials made of adsorbent mixes specially designed for all food and feed commodities, for fast cleanup within 30 seconds.
Using MultiSep® cleanup, the extract is applied on top of the column and passes through either by gravity or by applying vacuum or positive pressure. The purified extract, containing all the mycotoxins, passes through, whereas interferences adhere to the chemical packing.
A complete overview can be found below. Click on the item numbers for more information.
| Item No. | Product | Specificity | No. of Columns |
| MultiSep® | |||
|---|---|---|---|
| COCMU2211 | MultiSep® 211 Fum | Fumonisin B1, B2 & B3 | 25 |
| COCMU2216 | MultiSep® 216 | Trichothecenes, Type A & B | 25 |
| COCMU2224 | MultiSep® 224 AflaZon | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| COCMU2225 | MultiSep® 225 Trich | Trichothecenes, Type A & B | 25 |
| COCMU2226 | MultiSep® 226 AflaZon+ | Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol | 25 |
| COCMU22271,2 | MultiSep® 227 Trich+ | Trichothecenes, Type A & B | 25 |
| COCMU22282 | MultiSep® 228 AflaPat | Aflatoxins B1, B2, G1 & G2, Patulin | 25 |
| COCMU2229 | MultiSep® 229 Ochra | Ochratoxin A | 25 |
| COCMU2230 | MultiSep® 230 Niv | Nivalenol | 25 |
1AOAC official method
2Japanese Department of Agriculture approved
MycoSpin®
Cleanup Columns
Romer Labs MycoSpin® 400 multi-mycotoxin two-step cleanup column in a spin format was developed to be used for most regulated mycotoxins to shorten the time-consuming cleanup process. Multiple mycotoxins can be analyzed simultaneously by LC-MS/MS.
Highly accurate LC-MS/MS results are achieved by combining the MycoSpin® 400 cleanup with Biopure™ 13C isotope labeled standards.
Click on the item number below for more information.
| Item No. | Product | Specificity | No. of Columns |
| MycoSpin® | |||
|---|---|---|---|
| COCMY2400 | MycoSpin® 400 Multitoxin | Aflatoxin B1, B2, G1 & G2, Trichothecenes Type A & B, Zearalenone, Ochratoxin A, Fumonisin B1, B2 & B3 | 25 |
StarLineTM
Immunoaffinity Columns
The wide range of StarLine™ immunoaffinity columns (IAC) for regulated mycotoxins is based on a specific antibody-analyte binding technology. Toxin-specific antibodies coupled to gel particles capture the mycotoxins in the sample and release them after a washing step.
StarLine™ immunoaffinity columns are designed for simple and reliable cleanup before detection of mycotoxins in a broad range of different commodities. Those IAC can be used for the purification as well as concentration of mycotoxins prior to analysis by various techniques, such as HPLC, GC-MS, LC-MS, ELISA or direct fluorometry.
All Romer Labs IAC employ highly specific, monoclonal antibodies.
A complete overview can be found below. Click on the item numbers for more information.
| Item No. | Specificity | Format | No. of Columns |
| AflaStar™ FIT | |||
|---|---|---|---|
| COIAC1001 | Aflatoxins B1, B2, G1 & G2 | 1 mL | 25 |
| COIAC1501 | Aflatoxins B1, B2, G1 & G2 | 1 mL | 500 |
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| AflaStar™ R | |||
|---|---|---|---|
| COIAC1004 | Aflatoxins B1, B2, G1 & G2 | 3 mL | 25 |
| COIAC1504 | Aflatoxins B1, B2, G1 & G2 | 3 mL | 500 |
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| AflaStar™ M1 R | |||
|---|---|---|---|
| COIAC1005 | Aflatoxin M1 | 3 mL | 25 |
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| OchraStar™ FIT | |||
|---|---|---|---|
| COIAC2001 | Ochratoxin A | 1 mL | 25 |
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| OchraStar™ R | |||
|---|---|---|---|
| COIAC2004 | Ochratoxin A | 3 mL | 25 |
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| FumoniStar™ | |||
|---|---|---|---|
| COIAC3000 | Fumonisin B1, B2, B3 | 3 mL | 25 |
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| DONStar™ | |||
|---|---|---|---|
| COIAC5000 | Deoxynivalenol | 3 mL | 25 |
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| ZearaStar™ R | |||
|---|---|---|---|
| COIAC4004 | Zearalenone | 3 mL | 25 |
| Item No. | Quantitation Range | Limit of Detection | No. of Tests |
| T2/HT2 Star™ R | |||
|---|---|---|---|
| COIAC6004 | T2 Toxin, HT2 Toxin | 3 mL | 25 |
Additional Equipment
Romer Labs also offers additional equipment. For some analyzes (eg. aflatoxins) by HPLC-FLD, a derivatization of the analytes is necessary. Romer Labs provides equipment like the Romer Derivatization Unit (RDU, photochemical) and the Romer Cell (electrochemical) for post-column derivatization.
Romer Labs Evap System can be used for rapid evaporation of common organic solvents.
| Item No. | Description |
| RDUTM | |
|---|---|
| EQOLE1480 | Romer® Derivatization Unit |
| Item No. | Description |
| Romer® Cell | |
|---|---|
| EURCO1080 | Romer® Cell |
| Item No. | Description |
| Romer Evap® System | |
|---|---|
| EQOEV1030 | 12 Port, small, 115V |
| EQOEV1033 | 12 Port, large, 115V |
| EQOEV1040 | 24 Port, small, 115V |
| EQOEV1043 | 24 Port, large, 115V |
| EQOEV1031 | 12 Port, small, 230V |
| EQOEV1032 | 12 Port, large, 230V |
| EQOEV1041 | 24 Port, small, 230V |
| EQOEV1042 | 24 Port, large, 230V |
